气候变化领域集成服务门户(CCPortal): In situ genome sequencing resolves DNA sequence and structure in intact biological samples

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DOI	10.1126/science.aay3446
	In situ genome sequencing resolves DNA sequence and structure in intact biological samples
	Payne A.C.; Chiang Z.D.; Reginato P.L.; Mangiameli S.M.; Murray E.M.; Yao C.-C.; Markoulaki S.; Earl A.S.; Labade A.S.; Jaenisch R.; Church G.M.; Boyden E.S.; Buenrostro J.D.; Chen F.
发表日期	2021
ISSN	0036-8075
卷号	371 期号:6532
英文摘要	Understanding genome organization requires integration of DNA sequence and three-dimensional spatial context; however, existing genome-wide methods lack either base pair sequence resolution or direct spatial localization. Here, we describe in situ genome sequencing (IGS), a method for simultaneously sequencing and imaging genomes within intact biological samples. We applied IGS to human fibroblasts and early mouse embryos, spatially localizing thousands of genomic loci in individual nuclei. Using these data, we characterized parent-specific changes in genome structure across embryonic stages, revealed single-cell chromatin domains in zygotes, and uncovered epigenetic memory of global chromosome positioning within individual embryos. These results demonstrate how IGS can directly connect sequence and structure across length scales from single base pairs to whole organisms. © 2021 American Association for the Advancement of Science. All rights reserved.
英文关键词	biological method; DNA; genome; genomics; in situ measurement; sampling; ultrastructure; article; chromatin; chromosome positioning; DNA sequence; embryo; epigenetics; fibroblast; human; human cell; memory; mouse embryo; zygote; animal; cell nucleus; chemistry; chromosome; embryo development; genetic epigenesis; genetics; genome; high throughput sequencing; human chromosome; human genome; mammalian embryo; mouse; nucleotide sequence; single cell analysis; spatial analysis; ultrastructure; Animals; Base Sequence; Cell Nucleus; Chromatin; Chromosome Positioning; Chromosomes, Human; Chromosomes, Mammalian; Embryo, Mammalian; Embryonic Development; Epigenesis, Genetic; Fibroblasts; Genome; Genome, Human; High-Throughput Nucleotide Sequencing; Humans; Mice; Sequence Analysis, DNA; Single-Cell Analysis; Spatial Analysis
语种	英语
来源期刊	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/245017
作者单位	Media Arts and Sciences, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, United States; Broad Institute of MIT and Harvard University, Cambridge, MA 02142, United States; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, United States; Department of Biological Engineering, MIT, Cambridge, MA 02139, United States; Department of Genetics, Harvard Medical School, Boston, MA 02115, United States; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, United States; Department of Brain and Cognitive Sciences, MIT, Cambridge, MA 02139, United States; Whitehead Institute for Biomedical Research, Cambridge, MA 02139, United States; Department of Biology, MIT, Cambridge, MA 02139, United States; McGovern Institute, MIT, Cambridge, MA 02139, United States; Koch Institute, MIT, Cambridge, MA 02139, United States; Howard Hughes Medical Institute, Cambridge, MA 02139, United States; Centers for Neurobiological E...
推荐引用方式 GB/T 7714	Payne A.C.,Chiang Z.D.,Reginato P.L.,et al. In situ genome sequencing resolves DNA sequence and structure in intact biological samples[J],2021,371(6532).
APA	Payne A.C..,Chiang Z.D..,Reginato P.L..,Mangiameli S.M..,Murray E.M..,...&Chen F..(2021).In situ genome sequencing resolves DNA sequence and structure in intact biological samples.Science,371(6532).
MLA	Payne A.C.,et al."In situ genome sequencing resolves DNA sequence and structure in intact biological samples".Science 371.6532(2021).