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DOI	10.1126/science.6328659
	Simian sarcoma virus-transformed cells secrete a mitogen identical to platelet-derived growth factor
	Owen A.J.; Pantazis P.; Antoniades H.N.
发表日期	1984
ISSN	0036-8075
起始页码	54
结束页码	56
卷号	225 期号:4657
英文摘要	Normal rat kidney (NRK) cells transformed by simian sarcoma virus (SSV) release into the culture medium a biologically active mitogen with properties identical to those of human platelet-derived growth factor (PDGF). Like PDGF, the growth factor derived from SSV-NRK cells was shown to be stable to heat and sensitive to reducing agents. It was capable of inhibiting binding of labeled PDGF to the receptor on human fibroblasts. It also stimulated the phosphorylation of the same membrane protein (185 kilodaltons) in isolated plasma membranes from human fibroblasts. Immunoprecipitation of metabolically labeled proteins released by SSV-NRK cells showed that a 34-kilodalton protein was specifically precipitated by antiserum to PDGF. Upon reduction, this protein had a molecular size of 17 kilodaltons. PDGF has been shown to consist of two 14- to 18-kilodalton proteins linked by disulfide bonds.
英文关键词	mitogenic agent; platelet derived growth factor; animal experiment; cell transformation; etiology; heredity; in vitro study; kidney; kidney cell; nonhuman; oncogene; rat; simian sarcoma virus; Animal; Cell Transformation, Viral; Fibroblasts; Human; Mitogens; Platelet-Derived Growth Factor; Rats; Retroviridae; Sarcoma Virus, Woolly Monkey; Support, Non-U.S. Gov't; Support, U.S. Gov't, P.H.S.
语种	英语
来源期刊	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/244973
作者单位	Department of Nutrition, Harvard School of Public Health, Center for Blood Research, Boston, MA 02115
推荐引用方式 GB/T 7714	Owen A.J.,Pantazis P.,Antoniades H.N.. Simian sarcoma virus-transformed cells secrete a mitogen identical to platelet-derived growth factor[J],1984,225(4657).
APA	Owen A.J.,Pantazis P.,&Antoniades H.N..(1984).Simian sarcoma virus-transformed cells secrete a mitogen identical to platelet-derived growth factor.Science,225(4657).
MLA	Owen A.J.,et al."Simian sarcoma virus-transformed cells secrete a mitogen identical to platelet-derived growth factor".Science 225.4657(1984).