气候变化领域集成服务门户(CCPortal): Synthesis, debugging, and effects of synthetic chromosome consolidation: synVI and beyond

CCPortal

DOI	10.1126/science.aaf4831
	Synthesis, debugging, and effects of synthetic chromosome consolidation: synVI and beyond
	Mitchell L.A.; Wang A.; Stracquadanio G.; Kuang Z.; Wang X.; Yang K.; Richardson S.; Martin J.A.; Zhao Y.; Walker R.; Luo Y.; Dai H.; Dong K.; Tang Z.; Yang Y.; Cai Y.; Heguy A.; Ueberheide B.; Fenyö D.; Dai J.; Bader J.S.; Boeke J.D.
发表日期	2017
ISSN	0036-8075
卷号	355 期号:6329
英文摘要	We describe design, rapid assembly, and characterization of synthetic yeast Sc2.0 chromosome VI (synVI). A mitochondrial defect in the synVI strain mapped to synonymous coding changes within PRE4 (YFR050C), encoding an essential proteasome subunit; Sc2.0 coding changes reduced Pre4 protein accumulation by half. Completing Sc2.0 specifies consolidation of 16 synthetic chromosomes into a single strain. We investigated phenotypic, transcriptional, and proteomewide consequences of Sc2.0 chromosome consolidation in poly-synthetic strains. Another "bug" was discovered through proteomic analysis, associated with alteration of the HIS2 transcription start due to transfer RNA deletion and loxPsym site insertion. Despite extensive genetic alterations across 6% of the genome, no major global changes were detected in the poly-synthetic strain "omics" analyses. This work sets the stage for completion of a designer, synthetic eukaryotic genome. © 2017, American Association for the Advancement of Science. All rights reserved.
英文关键词	protein; protein Pre4; proteome; transcriptome; transfer RNA; unclassified drug; PRE4 protein, S cerevisiae; proteasome; Saccharomyces cerevisiae protein; chromosome; eukaryote; gene expression; genetic analysis; genome; phenotype; proteomics; Article; auxotrophy; chromosome; codon; controlled study; endoreduplication; fungal strain; gene interaction; gene mutation; nonhuman; open reading frame; phenotype; priority journal; proteomics; suppressor gene; synthetic chromosome VI; transcription initiation; transcriptomics; yeast; artificial cell; chemistry; chromosomal mapping; genetics; growth, development and aging; metabolism; procedures; Saccharomyces cerevisiae; synthetic biology; yeast artificial chromosome; Eukaryota; Artificial Cells; Chromosomes, Artificial, Yeast; Physical Chromosome Mapping; Proteasome Endopeptidase Complex; Proteomics; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Synthetic Biology
语种	英语
来源期刊	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/244794
作者单位	Department of Biochemistry and Molecular Pharmacology, New York University, Langone School of Medicine, New York, NY 10016, United States; Institute for Systems Genetics, New York University, Langone School of Medicine, New York, NY 10016, United States; High Throughput Biology Center, Johns Hopkins University School of Medicine, Baltimore, MD 21205, United States; Key Laboratory for Industrial Biocatalysis (Ministry of Education), Key Laboratory of Bioinformatics (Ministry of Education), Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing, 100084, China; Department of Biomedical Engineering, Institute of Genetic Medicine, Whiting School of Engineering, Johns Hopkins University, Baltimore, MD 21218, United States; School of Computer Science and Electronic Engineering, University of Essex, Wivenhoe Park, Colchester, CO4 3SQ, United Kingdom; Center for Synthetic and Systems Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, EH9 3JL, Un...
推荐引用方式 GB/T 7714	Mitchell L.A.,Wang A.,Stracquadanio G.,et al. Synthesis, debugging, and effects of synthetic chromosome consolidation: synVI and beyond[J],2017,355(6329).
APA	Mitchell L.A..,Wang A..,Stracquadanio G..,Kuang Z..,Wang X..,...&Boeke J.D..(2017).Synthesis, debugging, and effects of synthetic chromosome consolidation: synVI and beyond.Science,355(6329).
MLA	Mitchell L.A.,et al."Synthesis, debugging, and effects of synthetic chromosome consolidation: synVI and beyond".Science 355.6329(2017).