气候变化领域集成服务门户(CCPortal): Degradation of Gα by the N-end rule pathway

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DOI	10.1126/science.8073290
	Degradation of Gα by the N-end rule pathway
	Madura K.; Varshavsky A.
发表日期	1994
ISSN	0036-8075
起始页码	1454
结束页码	1458
卷号	265 期号:5177
英文摘要	The N-end rule relates the in vivo half-life of a protein to the identity of its amino-terminal residue. Overexpression of targeting components of the N-end rule pathway in Saccharomyces cerevisiae inhibited the growth of haploid but not diploid cells. This ploidy-dependent toxicity was shown to result from enhanced degradation of Gpa1, the α subunit. (Gα) of a heterotrimeric guanine nucleotide-binding protein (G protein) that regulates cell differentiation in response to mating pheromones. Sst2, a protein whose absence renders cells hypersensitive to pheromone, was essential for degradation of Gα but not other N-end rule substrates, suggesting the involvement of an indirect, or trans-, targeting mechanism. Gα degradation by the N-end rule pathway adds another regulatory dimension to the multitude of signaling functions mediated by G proteins.
英文关键词	Saccharomyces cerevisiae; guanine nucleotide binding protein; pheromone; article; nonhuman; priority journal; protein degradation; saccharomyces cerevisiae; signal transduction; Cell Division; Dipeptides; Fungal Proteins; GTP-Binding Proteins; Guanosine Triphosphate; Half-Life; Haploidy; Ligases; Saccharomyces cerevisiae; Support, U.S. Gov't, P.H.S.; Transformation, Genetic
语种	英语
来源期刊	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/244620
作者单位	Division of Biology, California Institute of Technology, Pasadena, CA 91125, United States; Department of Biochemistry, University of Medicine and Dentistry of New Jersey, R. W. Johnson Medical School, Piscataway, NJ 08854, United States
推荐引用方式 GB/T 7714	Madura K.,Varshavsky A.. Degradation of Gα by the N-end rule pathway[J],1994,265(5177).
APA	Madura K.,&Varshavsky A..(1994).Degradation of Gα by the N-end rule pathway.Science,265(5177).
MLA	Madura K.,et al."Degradation of Gα by the N-end rule pathway".Science 265.5177(1994).