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DOI	10.1126/science.1206445
	X-ray emission spectroscopy evidences a central carbon in the nitrogenase iron-molybdenum cofactor
	Lancaster K.M.; Roemelt M.; Ettenhuber P.; Hu Y.; Ribbe M.W.; Neese F.; Bergmann U.; DeBeer S.
发表日期	2011
ISSN	0036-8075
起始页码	974
结束页码	977
卷号	334 期号:6058
英文摘要	Nitrogenase is a complex enzyme that catalyzes the reduction of dinitrogen to ammonia. Despite insight from structural and biochemical studies, its structure and mechanism await full characterization. An iron-molybdenum cofactor (FeMoco) is thought to be the site of dinitrogen reduction, but the identity of a central atom in this cofactor remains unknown. Fe Kβ x-ray emission spectroscopy (XES) of intact nitrogenase MoFe protein, isolated FeMoco, and the FeMoco-deficient ΔnifB protein indicates that among the candidate atoms oxygen, nitrogen, and carbon, it is carbon that best fits the XES data. The experimental XES is supported by computational efforts, which show that oxidation and spin states do not affect the assignment of the central atom to C4-. Identification of the central atom will drive further studies on its role in catalysis.
英文关键词	carbon; molybdenum iron protein; nitrogen; nitrogenase; oxygen; ammonia; biochemical composition; carbon; catalysis; enzyme activity; iron; molybdenum; nitrogen; oxygen; protein; X-ray spectroscopy; article; atom; oxidation; priority journal; spectroscopy; X ray emission spectroscopy
语种	英语
来源期刊	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/244444
作者单位	Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, United States; Institut für Physikalische und Theoretische Chemie, Universität Bonn, D-53115 Bonn, Germany; Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, United States; Max-Planck-Institut für Bioanorganische Chemie, Stiftstrasse 34-36, D-45470 Mülheim an der Ruhr, Germany; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, United States
推荐引用方式 GB/T 7714	Lancaster K.M.,Roemelt M.,Ettenhuber P.,et al. X-ray emission spectroscopy evidences a central carbon in the nitrogenase iron-molybdenum cofactor[J],2011,334(6058).
APA	Lancaster K.M..,Roemelt M..,Ettenhuber P..,Hu Y..,Ribbe M.W..,...&DeBeer S..(2011).X-ray emission spectroscopy evidences a central carbon in the nitrogenase iron-molybdenum cofactor.Science,334(6058).
MLA	Lancaster K.M.,et al."X-ray emission spectroscopy evidences a central carbon in the nitrogenase iron-molybdenum cofactor".Science 334.6058(2011).