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DOI	10.1126/science.1234273
	Simultaneous femtosecond x-ray spectroscopy and diffraction of photosystem II at room temperature
	Kern J.; Alonso-Mori R.; Tran R.; Hattne J.; Gildea R.J.; Echols N.; Gloc̈kner C.; Hellmich J.; Laksmono H.; Sierra R.G.; Lassalle-Kaiser B.; Koroidov S.; Lampe A.; Han G.; Gul S.; DiFiore D.; Milathianaki D.; Fry A.R.; Miahnahri A.; Schafer D.W.; Messerschmidt M.; Seibert M.M.; Koglin J.E.; Sokaras D.; Weng T.-C.; Sellberg J.; Latimer M.J.; Grosse-Kunstleve R.W.; Zwart P.H.; White W.E.; Glatzel P.; Adams P.D.; Bogan M.J.; Williams G.J.; Boutet S.; Messinger J.; Zouni A.; Sauter N.K.; Yachandra V.K.; Bergmann U.; Yano J.
发表日期	2013
ISSN	0036-8075
起始页码	491
结束页码	495
卷号	340 期号:6131
英文摘要	Intense femtosecond x-ray pulses produced at the Linac Coherent Light Source (LCLS) were used for simultaneous x-ray diffraction (XRD) and x-ray emission spectroscopy (XES) of microcrystals of photosystem II (PS II) at room temperature. This method probes the overall protein structure and the electronic structure of the Mn4CaO5 cluster in the oxygen-evolving complex of PS II. XRD data are presented from both the dark state (S 1) and the first illuminated state (S2) of PS II. Our simultaneous XRD-XES study shows that the PS II crystals are intact during our measurements at the LCLS, not only with respect to the structure of PS II, but also with regard to the electronic structure of the highly radiation-sensitive Mn4CaO5 cluster, opening new directions for future dynamics studies.
英文关键词	calcium; manganese; metalloprotein; data acquisition; temperature; X-ray diffraction; X-ray spectroscopy; article; catalysis; crystallization; femtosecond laser; flame photometry; illumination; infrared spectroscopy; oxidation; photosystem II; priority journal; protein structure; roentgen spectroscopy; room temperature; X ray diffraction; x ray emission spectroscopy
语种	英语
来源期刊	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/244260
作者单位	Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, United States; Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, Menlo Park, CA 94025, United States; Max-Volmer-Laboratorium für Biophysikalische Chemie, Technische Universität, D-10623 Berlin, Germany; PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, United States; Institutionen För Kemi, Kemiskt Biologiskt Centrum, Umeå Universitet, Umeå, Sweden; Stanford Synchrotron Radiation Lightsource (SSRL), SLAC National Accelerator Laboratory, Menlo Park, CA 94025, United States; Department of Physics, AlbaNova, Stockholm University, S-106 91 Stockholm, Sweden; European Synchrotron Radiation Facility, F-38043 Grenoble Cedex 9, France; Synchrotron SOLEIL, F-91192 Gif-Sur-Yvette, France
推荐引用方式 GB/T 7714	Kern J.,Alonso-Mori R.,Tran R.,et al. Simultaneous femtosecond x-ray spectroscopy and diffraction of photosystem II at room temperature[J],2013,340(6131).
APA	Kern J..,Alonso-Mori R..,Tran R..,Hattne J..,Gildea R.J..,...&Yano J..(2013).Simultaneous femtosecond x-ray spectroscopy and diffraction of photosystem II at room temperature.Science,340(6131).
MLA	Kern J.,et al."Simultaneous femtosecond x-ray spectroscopy and diffraction of photosystem II at room temperature".Science 340.6131(2013).