气候变化领域集成服务门户(CCPortal): The Drosophila embryo at single-cell transcriptome resolution

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DOI	10.1126/science.aan3235
	The Drosophila embryo at single-cell transcriptome resolution
	Karaiskos N.; Wahle P.; Alles J.; Boltengagen A.; Ayoub S.; Kipar C.; Kocks C.; Rajewsky N.; Zinzen R.P.
发表日期	2017
ISSN	0036-8075
起始页码	194
结束页码	199
卷号	358 期号:6360
英文摘要	By the onset of morphogenesis, Drosophila embryos consist of about 6000 cells that express distinct gene combinations. Here, we used single-cell sequencing of precisely staged embryos and devised DistMap, a computational mapping strategy to reconstruct the embryo and to predict spatial gene expression approaching single-cell resolution. We produced a virtual embryo with about 8000 expressed genes per cell. Our interactive Drosophila Virtual Expression eXplorer (DVEX) database generates three-dimensional virtual in situ hybridizations and computes gene expression gradients. We used DVEX to uncover patterned expression of transcription factors and long noncoding RNAs, as well as signaling pathway components. Spatial regulation of Hippo signaling during early embryogenesis suggests a mechanism for establishing asynchronous cell proliferation. Our approach is suitable to generate transcriptomic blueprints for other complex tissues. © 2017, American Association for the Advancement of Science. All rights reserved.
英文关键词	long untranslated RNA; transcription factor; transcriptome; Drosophila protein; hpo protein, Drosophila; protein serine threonine kinase; signal peptide; transcriptome; cells and cell components; computer simulation; embryo; fly; gene expression; genetic analysis; hybridization; Article; cell communication; cell proliferation; developmental stage; Drosophila melanogaster; embryo; embryo development; gene expression; hippo signaling; in situ hybridization; nonhuman; priority journal; single cell analysis; transcriptomics; animal; cytology; embryology; genetics; nonmammalian embryo; procedures; signal transduction; single cell analysis; Animals; Cell Communication; Drosophila melanogaster; Drosophila Proteins; Embryo, Nonmammalian; In Situ Hybridization; Intracellular Signaling Peptides and Proteins; Protein-Serine-Threonine Kinases; Signal Transduction; Single-Cell Analysis; Transcriptome
语种	英语
来源期刊	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/244217
作者单位	Systems Biology of Gene Regulatory Elements, Berlin Institute for Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, 13125, Germany; Systems Biology of Neural Tissue Differentiation, BIMSB, MDC, Berlin, 13125, Germany
推荐引用方式 GB/T 7714	Karaiskos N.,Wahle P.,Alles J.,et al. The Drosophila embryo at single-cell transcriptome resolution[J],2017,358(6360).
APA	Karaiskos N..,Wahle P..,Alles J..,Boltengagen A..,Ayoub S..,...&Zinzen R.P..(2017).The Drosophila embryo at single-cell transcriptome resolution.Science,358(6360).
MLA	Karaiskos N.,et al."The Drosophila embryo at single-cell transcriptome resolution".Science 358.6360(2017).