气候变化领域集成服务门户(CCPortal): A ubiquitin ligase mediates target-directed microRNA decay independently of tailing and trimming

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DOI	10.1126/science.abc9546
	A ubiquitin ligase mediates target-directed microRNA decay independently of tailing and trimming
	Han J.; Lavigne C.A.; Jones B.T.; Zhang H.; Gillett F.; Mendell J.T.
发表日期	2020
ISSN	0036-8075
卷号	370 期号:6523
英文摘要	MicroRNAs (miRNAs) act in concert with Argonaute (AGO) proteins to repress target messenger RNAs. After AGO loading, miRNAs generally exhibit slow turnover. An important exception occurs when miRNAs encounter highly complementary targets, which can trigger a process called target-directed miRNA degradation (TDMD). During TDMD, miRNAs undergo tailing and trimming, suggesting that this is an important step in the decay mechanism. We identified a cullin-RING ubiquitin ligase (CRL), containing the substrate adaptor ZSWIM8, that mediates TDMD. The ZSWIM8 CRL interacts with AGO proteins, promotes TDMD in a tailing and trimming-independent manner, and regulates miRNA expression in multiple cell types. These findings suggest a model in which the ZSWIM8 ubiquitin ligase mediates TDMD by directing proteasomal decay of miRNA-containing complexes engaged with highly complementary targets. © 2020 American Association for the Advancement of Science. All rights reserved.
英文关键词	argonaute protein; microRNA; protein cullin RING ubiquitin ligase; protein ZSWIM8; ubiquitin protein ligase; unclassified drug; AGO2 protein, human; argonaute protein; long noncoding RNA OIP5, human; long untranslated RNA; microRNA; MIRN7 microRNA, human; ubiquitin protein ligase; ZSWIM8 protein, human; cell; cell component; enzyme activity; genetic engineering; physiological response; substrate; Article; enzyme substrate; gene control; gene expression; gene targeting; human; human cell; nonhuman; priority journal; protein analysis; protein protein interaction; RNA degradation; gene knockout; genetics; K-562 cell line; metabolism; RNA stability; Argonaute Proteins; Gene Knockout Techniques; Humans; K562 Cells; MicroRNAs; RNA Stability; RNA, Long Noncoding; Ubiquitin-Protein Ligases
语种	英语
来源期刊	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/243928
作者单位	Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, United States; Quantitative Biomedical Research Center, University of Texas Southwestern Medical Center, Dallas, TX 75390, United States; Department of Population and Data Sciences, University of Texas Southwestern Medical Center, Dallas, TX 75390, United States; Harold C. Simmons Comprehensive Cancer Center, Dallas, TX 75390, United States; Hamon Center for Regenerative Science and Medicine, Dallas, TX 75390, United States; University of Texas Southwestern Medical Center, Howard Hughes Medical Institute, Dallas, TX 75390, United States
推荐引用方式 GB/T 7714	Han J.,Lavigne C.A.,Jones B.T.,et al. A ubiquitin ligase mediates target-directed microRNA decay independently of tailing and trimming[J],2020,370(6523).
APA	Han J.,Lavigne C.A.,Jones B.T.,Zhang H.,Gillett F.,&Mendell J.T..(2020).A ubiquitin ligase mediates target-directed microRNA decay independently of tailing and trimming.Science,370(6523).
MLA	Han J.,et al."A ubiquitin ligase mediates target-directed microRNA decay independently of tailing and trimming".Science 370.6523(2020).