气候变化领域集成服务门户(CCPortal): Local protein synthesis is a ubiquitous feature of neuronal pre- And postsynaptic compartments

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DOI	10.1126/science.aau3644
	Local protein synthesis is a ubiquitous feature of neuronal pre- And postsynaptic compartments
	Hafner A.-S.; Donlin-Asp P.G.; Leitch B.; Herzog E.; Schuman E.M.
发表日期	2019
ISSN	0036-8075
卷号	364 期号:6441
英文摘要	There is ample evidence for localization of messenger RNAs (mRNAs) and protein synthesis in neuronal dendrites; however, demonstrations of these processes in presynaptic terminals are limited. We used expansion microscopy to resolve pre- and postsynaptic compartments in rodent neurons. Most presynaptic terminals in the hippocampus and forebrain contained mRNA and ribosomes. We sorted fluorescently labeled mouse brain synaptosomes and then sequenced hundreds of mRNA species present within excitatory boutons. After brief metabolic labeling, >30% of all presynaptic terminals exhibited a signal, providing evidence for ongoing protein synthesis. We tested different classic plasticity paradigms and observed distinct patterns of rapid pre- and/or postsynaptic translation. Thus, presynaptic terminals are translationally competent, and local protein synthesis is differentially recruited to drive compartment-specific phenotypes that underlie different forms of plasticity. © 2019 American Association for the Advancement of Science. All rights reserved.
英文关键词	3,5 dihydroxyphenylglycine; brain derived neurotrophic factor; chloramphenicol; messenger RNA; metabotropic receptor 1; metabotropic receptor 5; neurotrophin; puromycin; RNA 18S; RNA 28S; vesicular glutamate transporter 1; messenger RNA; Slc17a7 protein, mouse; vesicular glutamate transporter 1; brain; cell organelle; nervous system; phenotype; phenotypic plasticity; protein; RNA; adult; animal tissue; antibody labeling; Article; brain mitochondrion; cerebellum; controlled study; dendritic spine; electron microscopy; endoplasmic reticulum; fluorescence activated cell sorting; fluorescence activated synaptosome sorting; fluorescence in situ hybridization; fluorescence microscopy; hippocampus; immunodetection; in vitro study; microscopy; mouse; nerve cell plasticity; nerve ending; nonhuman; priority journal; protein degradation; protein homeostasis; protein localization; protein RNA binding; protein synthesis; rat; RNA sequence; stimulated emission depletion microscopy; animal; brain cortex; cell culture; cytology; dendrite; genetics; hypophysis; inbred rat strain; metabolism; mutant mouse strain; nerve cell; nerve cell plasticity; ribosome; synapse; Rodentia; Animals; Cells, Cultured; Cerebral Cortex; Dendrites; Mice; Mice, Mutant Strains; Neuronal Plasticity; Neurons; Pituitary Gland; Protein Biosynthesis; Rats; Rats, Inbred Strains; Ribosomes; RNA, Messenger; Synapses; Vesicular Glutamate Transport Protein 1
语种	英语
来源期刊	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/243912
作者单位	Max Planck Institute for Brain Research, Frankfurt, Germany; Department of Anatomy, School of Biomedical Sciences and the Brain Health Research Centre, University of Otago, Dunedin, New Zealand; Interdisciplinary Institute for Neuroscience, University of Bordeaux, UMR 5297, Bordeaux, F-33000, France; Interdisciplinary Institute for Neuroscience, CNRS, UMR 5297, Bordeaux, F-33000, France
推荐引用方式 GB/T 7714	Hafner A.-S.,Donlin-Asp P.G.,Leitch B.,et al. Local protein synthesis is a ubiquitous feature of neuronal pre- And postsynaptic compartments[J],2019,364(6441).
APA	Hafner A.-S.,Donlin-Asp P.G.,Leitch B.,Herzog E.,&Schuman E.M..(2019).Local protein synthesis is a ubiquitous feature of neuronal pre- And postsynaptic compartments.Science,364(6441).
MLA	Hafner A.-S.,et al."Local protein synthesis is a ubiquitous feature of neuronal pre- And postsynaptic compartments".Science 364.6441(2019).