气候变化领域集成服务门户(CCPortal): Architecture of the yeast small subunit processome

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DOI	10.1126/science.aal1880
	Architecture of the yeast small subunit processome
	Chaker-Margot M.; Barandun J.; Hunziker M.; Klinge S.
发表日期	2017
ISSN	0036-8075
卷号	355 期号:6321
英文摘要	The small subunit (SSU) processome, a large ribonucleoprotein particle, organizes the assembly of the eukaryotic small ribosomal subunit by coordinating the folding, cleavage, and modification of nascent pre-ribosomal RNA (rRNA). Here, we present the cryo-electron microscopy structure of the yeast SSU processome at 5.1-angstrom resolution. The structure reveals how large ribosome biogenesis complexes assist the 5' external transcribed spacer and U3 small nucleolar RNA in providing an intertwined RNA-protein assembly platform for the separate maturation of 18S rRNA domains. The strategic placement of a molecular motor at the center of the particle further suggests a mechanism for mediating conformational changes within this giant particle. This study provides a structural framework for a mechanistic understanding of eukaryotic ribosome assembly in the model organism Saccharomyces cerevisiae.
英文关键词	fungal RNA; guanosine triphosphatase; molecular motor; molecular scaffold; ribosome RNA; small nucleolar RNA; RNA 18S; RNA, U3 small nucleolar; Saccharomyces cerevisiae protein; biological development; cell organelle; electron microscopy; eukaryote; protein; RNA; yeast; Article; base pairing; biogenesis; controlled study; cryoelectron microscopy; crystal structure; fungal genetics; molecular interaction; nonhuman; priority journal; processome; protein assembly; protein cross linking; protein secondary structure; ribosome; ribosome subunit; RNA conformation; RNA degradation; Saccharomyces cerevisiae; small ribosomal subunit; beta sheet; chemistry; conformation; Saccharomyces cerevisiae; ultrastructure; Eukaryota; Saccharomyces cerevisiae; Cryoelectron Microscopy; Nucleic Acid Conformation; Protein Conformation, beta-Strand; Ribosome Subunits, Small, Eukaryotic; RNA, Fungal; RNA, Ribosomal; RNA, Ribosomal, 18S; RNA, Small Nucleolar; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins
语种	英语
来源期刊	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/243261
作者单位	Laboratory of Protein and Nucleic Acid Chemistry, Rockefeller University, New York, NY 10065, United States; Tri-Institutional Training Program in Chemical Biology, Rockefeller University, New York, NY 10065, United States
推荐引用方式 GB/T 7714	Chaker-Margot M.,Barandun J.,Hunziker M.,et al. Architecture of the yeast small subunit processome[J],2017,355(6321).
APA	Chaker-Margot M.,Barandun J.,Hunziker M.,&Klinge S..(2017).Architecture of the yeast small subunit processome.Science,355(6321).
MLA	Chaker-Margot M.,et al."Architecture of the yeast small subunit processome".Science 355.6321(2017).