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| DOI | 10.1126/science.aau0382 |
| Biosynthesis of the neurotoxin domoic acid in a bloom-forming diatom | |
| Brunson J.K.; McKinnie S.M.K.; Chekan J.R.; McCrow J.P.; Miles Z.D.; Bertrand E.M.; Bielinski V.A.; Luhavaya H.; Oborník M.; Smith G.J.; Hutchins D.A.; Allen A.E.; Moore B.S. | |
| 发表日期 | 2018 |
| ISSN | 0036-8075 |
| 起始页码 | 1356 |
| 结束页码 | 1358 |
| 卷号 | 361期号:6409 |
| 英文摘要 | Oceanic harmful algal blooms of Pseudo-nitzschia diatoms produce the potent mammalian neurotoxin domoic acid (DA). Despite decades of research, the molecular basis for its biosynthesis is not known. By using growth conditions known to induce DA production in Pseudo-nitzschia multiseries, we implemented transcriptome sequencing in order to identify DA biosynthesis genes that colocalize in a genomic four-gene cluster.We biochemically investigated the recombinant DA biosynthetic enzymes and linked their mechanisms to the construction of DA's diagnostic pyrrolidine skeleton, establishing a model for DA biosynthesis. Knowledge of the genetic basis for toxin production provides an orthogonal approach to bloom monitoring and enables study of environmental factors that drive oceanic DA production. © 2018 American Association for the Advancement of Science. All rights reserved. |
| 英文关键词 | domoic acid; domoic acid; kainic acid; neurotoxin; recombinant protein; algal bloom; biological production; diatom; DNA; gene; mammal; molecular analysis; organic acid; recombination; toxin; Article; biosynthesis; diatom; environmental factor; gene cluster; gene identification; gene structure; genetic transcription; nonhuman; priority journal; sequence analysis; toxin analysis; toxin structure; toxin synthesis; analogs and derivatives; chemistry; diatom; eutrophication; genetics; metabolism; multigene family; algae; Bacillariophyta; Mammalia; Pseudo-nitzschia; Pseudo-nitzschia multiseries; Diatoms; Eutrophication; Kainic Acid; Multigene Family; Neurotoxins; Recombinant Proteins |
| 语种 | 英语 |
| 来源期刊 | Science
 |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/243179 |
| 作者单位 | Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, San Diego, CA 92093, United States; Microbial and Environmental Genomics Group, J. Craig Venter Institute, San Diego, CA 92037, United States; Department of Biology, Dalhousie University, Halifax, NS B3H 4R2, Canada; Synthetic Biology and Bioenergy Group, J. Craig Venter Institute, San Diego, CA 92037, United States; Institute of Parasitology, University of South Bohemia, Biology Center CAS, Branišovská 31, Ceské Budejovice, 370 05, Czech Republic; Moss Landing Marine Laboratories, 8272 Moss Landing Road, Moss Landing, CA 95039, United States; Marine and Environmental Biology, Department of Biological Sciences, University of Southern California, San Diego, CA 90089, United States; Integrative Oceanography Division, Scripps Institution of Oceanography, University of California, San Diego, San Diego, CA 92037, United States; Skaggs School of Pharmacy and Pharmaceutical Sciences, ... |
| 推荐引用方式 GB/T 7714 | Brunson J.K.,McKinnie S.M.K.,Chekan J.R.,et al. Biosynthesis of the neurotoxin domoic acid in a bloom-forming diatom[J],2018,361(6409). |
| APA | Brunson J.K..,McKinnie S.M.K..,Chekan J.R..,McCrow J.P..,Miles Z.D..,...&Moore B.S..(2018).Biosynthesis of the neurotoxin domoic acid in a bloom-forming diatom.Science,361(6409). |
| MLA | Brunson J.K.,et al."Biosynthesis of the neurotoxin domoic acid in a bloom-forming diatom".Science 361.6409(2018). |
| 条目包含的文件 | 条目无相关文件。 | |||||
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