气候变化领域集成服务门户(CCPortal): Movement of retinal along the visual transduction path

CCPortal

DOI	10.1126/science.288.5474.2209
	Movement of retinal along the visual transduction path
	Borhan B.; Souto M.L.; Imai H.; Shichida Y.; Nakanishi K.
发表日期	2000
ISSN	0036-8075
起始页码	2209
结束页码	2212
卷号	288 期号:5474
英文摘要	Movement of the ligand/receptor complex in rhodopsin (Rh) has been traced. Bleaching of diazoketo rhodopsin (DK-Rh) containing 11-cis-3-diazo-4- oxoretinal yields batho-, lumi-, meta-I-, and meta-II-Rh intermediates corresponding to those of native Rh but at lower temperatures. Photoaffinity labeling of DK-Rh and these bleaching intermediates shows that the ionone ring cross-links to tryptophan-265 on helix F in DK-Rh and batho-Rh, and to alanine-169 on helix D in lumi-, meta-I-, and meta-II-Rh intermediates. It is likely that these movements involving a flip-over of the chromophoric ring trigger changes in cytoplasmic membrane loops resulting in heterotrimeric guanine nucleotide-binding protein (G protein) activation.
英文关键词	11 cis 3 diazo 4 oxoretinal; alanine; bathorhodopsin; glycerol; guanine nucleotide binding protein; ionone derivative; opsin; retinal; retinol derivative; rhodopsin; transducin; tryptophan; unclassified drug; article; chemical reaction; chemical structure; cis trans isomerism; conformational transition; membrane structure; priority journal; protein localization; reaction analysis; signal transduction; structure analysis; temperature dependence; visual system; Affinity Labels; Azo Compounds; Binding Sites; Circular Dichroism; Heterotrimeric GTP-Binding Proteins; Ligands; Light; Models, Molecular; Photolysis; Phototransduction; Protein Binding; Protein Conformation; Protein Structure, Secondary; Retinaldehyde; Rhodopsin; Rod Outer Segments; Stereoisomerism; Temperature
语种	英语
来源期刊	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/243097
作者单位	Department of Chemistry, Columbia University, New York, NY 10027, United States; Department of Biophysics, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan
推荐引用方式 GB/T 7714	Borhan B.,Souto M.L.,Imai H.,et al. Movement of retinal along the visual transduction path[J],2000,288(5474).
APA	Borhan B.,Souto M.L.,Imai H.,Shichida Y.,&Nakanishi K..(2000).Movement of retinal along the visual transduction path.Science,288(5474).
MLA	Borhan B.,et al."Movement of retinal along the visual transduction path".Science 288.5474(2000).