气候变化领域集成服务门户(CCPortal): Monosomes actively translate synaptic mRNAs in neuronal processes

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DOI	10.1126/science.aay4991
	Monosomes actively translate synaptic mRNAs in neuronal processes
	Biever A.; Glock C.; Tushev G.; Ciirdaeva E.; Dalmay T.; Langer J.D.; Schuman E.M.
发表日期	2020
ISSN	0036-8075
卷号	367 期号:6477
英文摘要	To accommodate their complex morphology, neurons localize messenger RNAs (mRNAs) and ribosomes near synapses to produce proteins locally. However, a relative paucity of polysomes (considered the active sites of translation) detected in electron micrographs of neuronal processes has suggested a limited capacity for local protein synthesis. In this study, we used polysome profiling together with ribosome footprinting of microdissected rodent synaptic regions to reveal a surprisingly high number of dendritic and/or axonal transcripts preferentially associated with monosomes (single ribosomes). Furthermore, the neuronal monosomes were in the process of active protein synthesis. Most mRNAs showed a similar translational status in the cell bodies and neurites, but some transcripts exhibited differential ribosome occupancy in the compartments. Monosome-preferring transcripts often encoded high-abundance synaptic proteins. Thus, monosome translation contributes to the local neuronal proteome. © 2020 American Association for the Advancement of Science. All rights reserved.
英文关键词	proteome; messenger RNA; proteome; cell; cell component; protein; proteomics; RNA; adult; animal cell; animal tissue; Article; controlled study; dendrite; monosome; mouse; neuropil; nonhuman; priority journal; protein synthesis; rat; ribosome; RNA transcription; RNA translation; synapse; animal; C57BL mouse; genetics; male; metabolism; mutant mouse strain; polysome; protein synthesis; ribosome; synapse; Rodentia; Animals; Male; Mice; Mice, Inbred C57BL; Mice, Mutant Strains; Neuropil; Polyribosomes; Protein Biosynthesis; Proteome; Ribosomes; RNA, Messenger; Synapses
语种	英语
来源期刊	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/243042
作者单位	Max Planck Institute for Brain Research, Frankfurt, Germany; Max Planck Institute of Biophysics, Frankfurt, Germany
推荐引用方式 GB/T 7714	Biever A.,Glock C.,Tushev G.,et al. Monosomes actively translate synaptic mRNAs in neuronal processes[J],2020,367(6477).
APA	Biever A..,Glock C..,Tushev G..,Ciirdaeva E..,Dalmay T..,...&Schuman E.M..(2020).Monosomes actively translate synaptic mRNAs in neuronal processes.Science,367(6477).
MLA	Biever A.,et al."Monosomes actively translate synaptic mRNAs in neuronal processes".Science 367.6477(2020).