气候变化领域集成服务门户(CCPortal): Hierarchical transitions and fractal wrinkling drive bacterial pellicle morphogenesis

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DOI	10.1073/pnas.2023504118
	Hierarchical transitions and fractal wrinkling drive bacterial pellicle morphogenesis
	Qin B.; Fei C.; Wang B.; Stone H.A.; Wingreen N.S.; Bassler B.L.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:20
英文摘要	Bacterial cells can self-organize into structured communities at fluid–fluid interfaces. These soft, living materials composed of cells and extracellular matrix are called pellicles. Cells residing in pellicles garner group-level survival advantages such as increased antibiotic resistance. The dynamics of pellicle formation and, more generally, how complex morphologies arise from active biomaterials confined at interfaces are not well understood. Here, using Vibrio cholerae as our model organism, a custom-built adaptive stereo microscope, fluorescence imaging, mechanical theory, and simulations, we report a fractal wrinkling morphogenesis program that differs radically from the well-known coalescence of wrinkles into folds that occurs in passive thin films at fluid–fluid interfaces. Four stages occur: growth of founding colonies, onset of primary wrinkles, development of secondary curved ridge instabilities, and finally the emergence of a cascade of finer structures with fractal-like scaling in wavelength. The time evolution of pellicle formation depends on the initial heterogeneity of the film microstructure. Changing the starting bacterial seeding density produces three variations in the sequence of morphogenic stages, which we term the bypass, crystalline, and incomplete modes. Despite these global architectural transitions, individual microcolonies remain spatially segregated, and thus, the community maintains spatial and genetic heterogeneity. Our results suggest that the memory of the original microstructure is critical in setting the morphogenic dynamics of a pellicle as an active biomaterial. © 2021 National Academy of Sciences. All rights reserved.
语种	英语
scopus关键词	algorithm; Article; bacterial cell; bacterial growth; bacterial strain; bacterium colony; controlled study; evolution; extracellular matrix; genetic heterogeneity; molecular dynamics; morphogenesis; nonhuman; surface area; Vibrio cholerae
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238921
作者单位	Department of Molecular Biology, Princeton University, Princeton, NJ 08544, United States; Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ 08544, United States; Lewis–Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, United States; Howard Hughes Medical Institute, Chevy Chase, MD 20815, United States
推荐引用方式 GB/T 7714	Qin B.,Fei C.,Wang B.,et al. Hierarchical transitions and fractal wrinkling drive bacterial pellicle morphogenesis[J],2021,118(20).
APA	Qin B.,Fei C.,Wang B.,Stone H.A.,Wingreen N.S.,&Bassler B.L..(2021).Hierarchical transitions and fractal wrinkling drive bacterial pellicle morphogenesis.Proceedings of the National Academy of Sciences of the United States of America,118(20).
MLA	Qin B.,et al."Hierarchical transitions and fractal wrinkling drive bacterial pellicle morphogenesis".Proceedings of the National Academy of Sciences of the United States of America 118.20(2021).