气候变化领域集成服务门户(CCPortal): The immune response is a critical regulator of zebrafish retinal pigment epithelium regeneration

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DOI	10.1073/pnas.2017198118
	The immune response is a critical regulator of zebrafish retinal pigment epithelium regeneration
	Leach L.L.; Hanovice N.J.; George S.M.; Gabriel A.E.; Gross J.M.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:21
英文摘要	Loss of the retinal pigment epithelium (RPE) because of dysfunction or disease can lead to blindness in humans. Harnessing the intrinsic ability of the RPE to self-repair is an attractive therapeutic strategy; however, mammalian RPE is limited in its regenerative capacity. Zebrafish possess tremendous intrinsic regenerative potential in ocular tissues, including the RPE, but little is known about the mechanisms driving RPE regeneration. Here, utilizing transgenic and mutant zebrafish lines, pharmacological manipulations, transcriptomics, and imaging analyses, we identified elements of the immune response as critical mediators of intrinsic RPE regeneration. After genetic ablation, the RPE express immune-related genes, including leukocyte recruitment factors such as interleukin 34. We demonstrate that macrophage/microglia cells are responsive to RPE damage and that their function is required for the timely progression of the regenerative response. These data identify the molecular and cellular underpinnings of RPE regeneration and hold significant potential for translational approaches aimed toward promoting a pro-regenerative environment in mammalian RPE. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	inflammation; macrophage; microglia; regeneration; Retinal pigment epithelium
语种	英语
scopus关键词	interleukin 34; adult; animal cell; animal experiment; animal tissue; apoptosis; Article; cell function; cell proliferation; controlled study; embryo; gene expression; immune response; macrophage; microglia; mutant; nonhuman; retinal pigment epithelium; tissue injury; tissue regeneration; transcriptomics; transgenic zebrafish; TUNEL assay; upregulation; vitreoretinopathy
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238915
作者单位	Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, United States; Department of Developmental Biology, University, Pittsburgh School of Medicine, Pittsburgh, PA 15213, United States
推荐引用方式 GB/T 7714	Leach L.L.,Hanovice N.J.,George S.M.,et al. The immune response is a critical regulator of zebrafish retinal pigment epithelium regeneration[J],2021,118(21).
APA	Leach L.L.,Hanovice N.J.,George S.M.,Gabriel A.E.,&Gross J.M..(2021).The immune response is a critical regulator of zebrafish retinal pigment epithelium regeneration.Proceedings of the National Academy of Sciences of the United States of America,118(21).
MLA	Leach L.L.,et al."The immune response is a critical regulator of zebrafish retinal pigment epithelium regeneration".Proceedings of the National Academy of Sciences of the United States of America 118.21(2021).