气候变化领域集成服务门户(CCPortal): Retrograde sulfur flow from glucosinolates to cysteine in Arabidopsis thaliana

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DOI	10.1073/pnas.2017890118
	Retrograde sulfur flow from glucosinolates to cysteine in Arabidopsis thaliana
	Sugiyama R.; Li R.; Kuwahara A.; Nakabayashi R.; Sotta N.; Mori T.; Ito T.; Ohkama-Ohtsu N.; Fujiwara T.; Saito K.; Nakano R.T.; Bednarek P.; Hirai M.Y.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:22
英文摘要	Specialized (secondary) metabolic pathways in plants have long been considered one-way routes of leading primary metabolite precursors to bioactive end products. Conversely, endogenous degradation of such “end” products in plant tissues has been observed following environmental stimuli, including nutrition stress. Therefore, it is of general interest whether specialized metabolites can be reintegrated into primary metabolism to recover the invested resources, especially in the case of nitrogen- or sulfur-rich compounds. Here, we demonstrate that endogenous glucosinolates (GLs), a class of sulfur-rich plant metabolites, are exploited as a sulfur source by the reallocation of sulfur atoms to primary metabolites such as cysteine in Arabidopsis thaliana. Tracer experiments using 34S- or deuterium-labeled GLs depicted the catabolic processing of GL breakdown products in which sulfur is mobilized from the thioglucoside group in GL molecules, potentially accompanied by the release of the sulfate group. Moreover, we reveal that beta-glucosidases BGLU28 and BGLU30 are the major myrosinases that initiate sulfur reallocation by hydrolyzing particular GL species, conferring sulfur deficiency tolerance in A. thaliana, especially during early development. The results delineate the physiological function of GL as a sulfur reservoir, in addition to their well-known functions as defense chemicals. Overall, our findings demonstrate the bidirectional interaction between primary and specialized metabolism, which enhances our understanding of the underlying metabolic mechanisms via which plants adapt to their environments. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Glucosinolate; Specialized metabolism; Stress response; Sulfur
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238905
作者单位	Metabolic Systems Research Team, RIKEN Center for Sustainable Resource Science, Yokohama, 230-0045, Japan; College of Life Sciences, Northeast Agricultural University, Harbin, 150030, China; Metabolomics Research Group, RIKEN Center for Sustainable Resource Science, Yokohama, 230-0045, Japan; Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo, 113-8657, Japan; Institute of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, 183-8509, Japan; Institute of Global Innovation research, Tokyo University of Agriculture and Technology, Tokyo, 183-8509, Japan; Plant Molecular Science Center, Chiba University, Chiba, 260-8675, Japan; Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, Cologne, 50829, Germany; Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, 61-704, Poland; Department of Pharmacy, National University of Singapore, Singapore, 117544, Singapore; Departmen...
推荐引用方式 GB/T 7714	Sugiyama R.,Li R.,Kuwahara A.,et al. Retrograde sulfur flow from glucosinolates to cysteine in Arabidopsis thaliana[J],2021,118(22).
APA	Sugiyama R..,Li R..,Kuwahara A..,Nakabayashi R..,Sotta N..,...&Hirai M.Y..(2021).Retrograde sulfur flow from glucosinolates to cysteine in Arabidopsis thaliana.Proceedings of the National Academy of Sciences of the United States of America,118(22).
MLA	Sugiyama R.,et al."Retrograde sulfur flow from glucosinolates to cysteine in Arabidopsis thaliana".Proceedings of the National Academy of Sciences of the United States of America 118.22(2021).