气候变化领域集成服务门户(CCPortal): Photosynthesis-independent production of reactive oxygen species in the rice bundle sheath during high light is mediated by NADPH oxidase

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DOI	10.1073/pnas.2022702118
	Photosynthesis-independent production of reactive oxygen species in the rice bundle sheath during high light is mediated by NADPH oxidase
	Xiong H.; Hua L.; Reyna-Llorens I.; Shi Y.; Chen K.-M.; Smirnoff N.; Kromdijk J.; Hibberd J.M.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:25
英文摘要	When exposed to high light, plants produce reactive oxygen species (ROS). In Arabidopsis thaliana, local stress such as excess heat or light initiates a systemic ROS wave in phloem and xylem cells dependent on NADPH oxidase/respiratory burst oxidase homolog (RBOH) proteins. In the case of excess light, although the initial local accumulation of ROS preferentially takes place in bundle-sheath strands, little is known about how this response takes place. Using rice and the ROS probes diaminobenzidine and 2',7'-dichlorodihydrofluorescein diacetate, we found that, after exposure to high light, ROS were produced more rapidly in bundle-sheath strands than mesophyll cells. This response was not affected either by CO2 supply or photorespiration. Consistent with these findings, deep sequencing of messenger RNA (mRNA) isolated from mesophyll or bundle-sheath strands indicated balanced accumulation of transcripts encoding all major components of the photosynthetic apparatus. However, transcripts encoding several isoforms of the superoxide/H2O2-producing enzyme NADPH oxidase were more abundant in bundle-sheath strands than mesophyll cells. ROS production in bundle-sheath strands was decreased in mutant alleles of the bundle-sheath strand preferential isoform of OsRBOHA and increased when it was overexpressed. Despite the plethora of pathways able to generate ROS in response to excess light, NADPH oxidase-mediated accumulation of ROS in the rice bundle-sheath strand was detected in etiolated leaves lacking chlorophyll. We conclude that photosynthesis is not necessary for the local ROS response to high light but is in part mediated by NADPH oxidase activity. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Bundle sheath strands; High light; Reactive oxygen species; Rice
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238885
作者单位	Department of Plant Sciences, University of Cambridge, Cambridge, CB2 3EA, United Kingdom; State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest AandF University, Yangling, 712100, China; College of Life Sciences, Northwest AandF University, Yangling, 712100, China; College of Life and Environmental Sciences, University of Exeter, Exeter, EX4 4QD, United Kingdom
推荐引用方式 GB/T 7714	Xiong H.,Hua L.,Reyna-Llorens I.,et al. Photosynthesis-independent production of reactive oxygen species in the rice bundle sheath during high light is mediated by NADPH oxidase[J],2021,118(25).
APA	Xiong H..,Hua L..,Reyna-Llorens I..,Shi Y..,Chen K.-M..,...&Hibberd J.M..(2021).Photosynthesis-independent production of reactive oxygen species in the rice bundle sheath during high light is mediated by NADPH oxidase.Proceedings of the National Academy of Sciences of the United States of America,118(25).
MLA	Xiong H.,et al."Photosynthesis-independent production of reactive oxygen species in the rice bundle sheath during high light is mediated by NADPH oxidase".Proceedings of the National Academy of Sciences of the United States of America 118.25(2021).