气候变化领域集成服务门户(CCPortal): Connecting structure and function from organisms to molecules in small-animal symbioses through chemo-histo-tomography

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DOI	10.1073/pnas.2023773118
	Connecting structure and function from organisms to molecules in small-animal symbioses through chemo-histo-tomography
	Geier B.; Oetjen J.; Ruthensteiner B.; Polikarpov M.; Gruber-Vodicka H.R.; Liebeke M.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:27
英文摘要	Our understanding of metabolic interactions between small symbiotic animals and bacteria or parasitic eukaryotes that reside within their bodies is extremely limited. This gap in knowledge originates from a methodological challenge, namely to connect histological changes in host tissues induced by beneficial and parasitic (micro)organisms to the underlying metabolites. We addressed this challenge and developed chemo-histo-tomography (CHEMHIST), a culture-independent approach to connect anatomic structure and metabolic function in millimeter-sized symbiotic animals. CHEMHIST combines chemical imaging of metabolites based on mass spectrometry imaging (MSI) and microanatomy-based micro-computed X-ray tomography (micro-CT) on the same animal. Both high-resolution MSI and micro-CT allowed us to correlate the distribution of metabolites to the same animal's three-dimensional (3D) histology down to submicrometer resolutions. Our protocol is compatible with tissue-specific DNA sequencing and fluorescence in situ hybridization for the taxonomic identification and localization of the associated micro(organisms). Building CHEMHIST upon in situ imaging, we sampled an earthworm from its natural habitat and created an interactive 3D model of its physical and chemical interactions with bacteria and parasitic nematodes in its tissues. Combining MSI and micro-CT, we present a methodological groundwork for connecting metabolic and anatomic phenotypes of small symbiotic animals that often represent keystone species for ecosystem functioning. © This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).
英文关键词	3D reconstruction; Metabolomics; Multimodal mass spectrometry imaging; Symbiosis; X-ray micro-CT imaging
语种	英语
scopus关键词	article; chemical imaging; chemical interaction; controlled study; DNA sequencing; earthworm; fluorescence in situ hybridization; habitat; histology; histopathology; human tissue; keystone species; mass spectrometry; metabolomics; micro-computed tomography; microorganism; nematode; nonhuman; phenotype; physical model; structure activity relation; symbiosis; taxonomic identification; x-ray tomography
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238875
作者单位	Max Planck Institute for Marine Microbiology, Bremen, 28359, Germany; MALDI Imaging Lab, University of Bremen, Bremen, 28334, Germany; SNSB, The Bavarian State Collection of Zoology, Munich, 81247, Germany; European Molecular Biology Laboratory, Hamburg Unit c/o Deutsches Elektronen-Synchrotron, Hamburg, 22607, Germany
推荐引用方式 GB/T 7714	Geier B.,Oetjen J.,Ruthensteiner B.,et al. Connecting structure and function from organisms to molecules in small-animal symbioses through chemo-histo-tomography[J],2021,118(27).
APA	Geier B.,Oetjen J.,Ruthensteiner B.,Polikarpov M.,Gruber-Vodicka H.R.,&Liebeke M..(2021).Connecting structure and function from organisms to molecules in small-animal symbioses through chemo-histo-tomography.Proceedings of the National Academy of Sciences of the United States of America,118(27).
MLA	Geier B.,et al."Connecting structure and function from organisms to molecules in small-animal symbioses through chemo-histo-tomography".Proceedings of the National Academy of Sciences of the United States of America 118.27(2021).