气候变化领域集成服务门户(CCPortal): Replication-dependent histone biosynthesis is coupled to cell-cycle commitment

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DOI	10.1073/pnas.2100178118
	Replication-dependent histone biosynthesis is coupled to cell-cycle commitment
	Armstrong C.; Spencer S.L.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:31
英文摘要	The current model of replication-dependent (RD) histone biosynthesis posits that RD histone gene expression is coupled to DNA replication, occurring only in S phase of the cell cycle once DNA synthesis has begun. However, several key factors in the RD histone biosynthesis pathway are up-regulated by E2F or phosphorylated by CDK2, suggesting these processes may instead begin much earlier, at the point of cell-cycle commitment. In this study, we use both fixed- and live-cell imaging of human cells to address this question, revealing a hybrid model in which RD histone biosynthesis is first initiated in G1, followed by a strong increase in histone production in S phase of the cell cycle. This suggests a mechanism by which cells that have committed to the cell cycle build up an initial small pool of RD histones to be available for the start of DNA replication, before producing most of the necessary histones required in S phase. Thus, a clear distinction exists at completion of mitosis between cells that are born with the intention of proceeding through the cell cycle and replicating their DNA and cells that have chosen to exit the cell cycle and have no immediate need for histone synthesis. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Histone locus body; NPAT; Replication-dependent histone; Restriction point; SLBP
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238840
作者单位	Department of Biochemistry, University of Colorado, Boulder, CO 80303, United States; BioFrontiers Institute, University of Colorado, Boulder, CO 80309, United States
推荐引用方式 GB/T 7714	Armstrong C.,Spencer S.L.. Replication-dependent histone biosynthesis is coupled to cell-cycle commitment[J],2021,118(31).
APA	Armstrong C.,&Spencer S.L..(2021).Replication-dependent histone biosynthesis is coupled to cell-cycle commitment.Proceedings of the National Academy of Sciences of the United States of America,118(31).
MLA	Armstrong C.,et al."Replication-dependent histone biosynthesis is coupled to cell-cycle commitment".Proceedings of the National Academy of Sciences of the United States of America 118.31(2021).