气候变化领域集成服务门户(CCPortal): The Sw5a gene confers resistance to ToLCNDV and triggers an HR response after direct AC4 effector recognition

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DOI	10.1073/pnas.2101833118
	The Sw5a gene confers resistance to ToLCNDV and triggers an HR response after direct AC4 effector recognition
	Sharma N.; Sahu P.P.; Prasad A.; Muthamilarasan M.; Waseem M.; Khan Y.; Thakur J.K.; Chakraborty S.; Prasad M.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:33
英文摘要	Several attempts have been made to identify antiviral genes against Tomato leaf curl New Delhi virus (ToLCNDV) and related viruses. This has led to the recognition of Ty genes (Ty1-Ty6), which have been successful in developing virus-resistant crops to some extent. Owing to the regular appearance of resistance-breaking strains of these viruses, it is important to identify genes related to resistance. In the present study, we identified a ToLCNDV resistance (R) gene, SlSw5a, in a ToLCNDV-resistant tomato cultivar, H-88-78-1, which lacks the known Ty genes. The expression of SlSw5a is controlled by the transcription factor SlMyb33, which in turn is regulated by microRNA159 (sly-miR159). Virus-induced gene silencing of either SlSw5a or SlMyb33 severely increases the disease symptoms and viral titer in leaves of resistant cultivar. Moreover, in SlMyb33-silenced plants, the relative messenger RNA level of SlSw5a was reduced, suggesting SlSw5a is downstream of the sly-miR159-SlMyb33 module. We also demonstrate that SlSw5a interacts physically with ToLCNDV-AC4 (viral suppressor of RNA silencing) to trigger a hypersensitive response (HR) and generate reactive oxygen species at infection sites to limit the spread of the virus. The “RTSK” motif in the AC4 C terminus is important for the interaction, and its mutation completely abolishes the interaction with Sw5a and HR elicitation. Overall, our research reports an R gene against ToLCNDV and establishes a connection between the upstream miR159-Myb33 module and its downstream target Sw5a to activate HR in the tomato, resulting in geminivirus resistance. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Hypersensitive response; MicroRNAs; Myb transcription factor; Sw5 gene; Tomato leaf curl virus
语种	英语
scopus关键词	AC4 protein; messenger RNA; microRNA; microRNA 159; reactive oxygen metabolite; unclassified drug; viral protein; allergic reaction; Article; carboxy terminal sequence; controlled study; disease course; Geminiviridae; gene control; gene expression; gene interaction; gene silencing; nonhuman; plant gene; plant leaf; protein analysis; Sw5a gene; tomato; tomato leaf curl New Delhi virus; virus load; virus mutation; virus resistance
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238828
作者单位	Laboratory of Plant Virology, National Institute of Plant Genome Research, New Delhi, 110067, India; School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India; School of Computational and Integrative Sciences, Jawaharlal Nehru University, New Delhi, 110067, India; Plant Transcription Regulation, International Centre for Genetic Engineering and Biotechnology, New Delhi, 110067, India; Global Change Research Institute of the Czech Academy of Sciences, Brno, 60300, Czech Republic; Repository of Tomato Genomics Resources, Department of Plant Sciences, University of Hyderabad, Telangana, Hyderabad, 500046, India; Department of Biotechnology, Inland Norway University of Applied Sciences, Hamar, 2418, Norway
推荐引用方式 GB/T 7714	Sharma N.,Sahu P.P.,Prasad A.,et al. The Sw5a gene confers resistance to ToLCNDV and triggers an HR response after direct AC4 effector recognition[J],2021,118(33).
APA	Sharma N..,Sahu P.P..,Prasad A..,Muthamilarasan M..,Waseem M..,...&Prasad M..(2021).The Sw5a gene confers resistance to ToLCNDV and triggers an HR response after direct AC4 effector recognition.Proceedings of the National Academy of Sciences of the United States of America,118(33).
MLA	Sharma N.,et al."The Sw5a gene confers resistance to ToLCNDV and triggers an HR response after direct AC4 effector recognition".Proceedings of the National Academy of Sciences of the United States of America 118.33(2021).