气候变化领域集成服务门户(CCPortal): Recombination-independent recognition of DNA homology for meiotic silencing in Neurospora crassa

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DOI	10.1073/pnas.2108664118
	Recombination-independent recognition of DNA homology for meiotic silencing in Neurospora crassa
	Rhoades N.; Nguyen T.-S.; Witz G.; Cecere G.; Hammond T.; Mazur A.K.; Gladyshev E.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:33
英文摘要	The pairing of homologous chromosomes represents a critical step of meiosis in nearly all sexually reproducing species. In many organisms, pairing involves chromosomes that remain apparently intact. The mechanistic nature of homology recognition at the basis of such pairing is unknown. Using “meiotic silencing by unpaired DNA” (MSUD) as a model process, we demonstrate the existence of a cardinally different approach to DNA homology recognition in meiosis. The main advantage of MSUD over other experimental systems lies in its ability to identify any relatively short DNA fragment lacking a homologous allelic partner. Here, we show that MSUD does not rely on the canonical mechanism of meiotic recombination, yet it is promoted by REC8, a conserved component of the meiotic cohesion complex. We also show that certain patterns of interspersed homology are recognized as pairable during MSUD. Such patterns need to be colinear and must contain short tracts of sequence identity spaced apart at 21 or 22 base pairs. By using these periodicity values as a guiding parameter in all-atom molecular modeling, we discover that homologous DNA molecules can pair by forming quadruplex-based contacts with an interval of 2.5 helical turns. This process requires right-handed plectonemic coiling and additional conformational changes in the intervening double-helical segments. Our results 1) reconcile genetic and biophysical evidence for the existence of direct homologous double-stranded DNA (dsDNA)-dsDNA pairing, 2) identify a role for this process in initiating RNA interference, and 3) suggest that chromosomes can be cross-matched by a precise mechanism that operates on intact dsDNA molecules. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	DNA; Homology recognition; Meiosis; MSUD; Neurospora
语种	英语
scopus关键词	double stranded DNA; Article; conformational transition; controlled study; DNA helix; gene locus; gene sequence; gene silencing; meiosis; meiotic recombination; molecular model; Neurospora; Neurospora crassa; nonhuman; periodicity; RNA interference; sequence homology
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238824
作者单位	School of Biological Sciences, Illinois State University, Normal, IL 61761, United States; Group Fungal Epigenomics, Department of Mycology, Institut Pasteur, Paris, 75015, France; Microscopy Imaging Center, Science IT Support, Mathematical Institute, University of Bern, Bern, 3005, Switzerland; Mechanisms of Epigenetic Inheritance, Department of Developmental and Stem Cell Biology, UMR 3738, CNRS, Institut Pasteur, Paris, 75015, France; Laboratoire de Biochimie Théorique, UPR 9080 CNRS, Université de Paris, Paris, 75005, France
推荐引用方式 GB/T 7714	Rhoades N.,Nguyen T.-S.,Witz G.,et al. Recombination-independent recognition of DNA homology for meiotic silencing in Neurospora crassa[J],2021,118(33).
APA	Rhoades N..,Nguyen T.-S..,Witz G..,Cecere G..,Hammond T..,...&Gladyshev E..(2021).Recombination-independent recognition of DNA homology for meiotic silencing in Neurospora crassa.Proceedings of the National Academy of Sciences of the United States of America,118(33).
MLA	Rhoades N.,et al."Recombination-independent recognition of DNA homology for meiotic silencing in Neurospora crassa".Proceedings of the National Academy of Sciences of the United States of America 118.33(2021).