气候变化领域集成服务门户(CCPortal): Dynamic localization of a helper NLR at the plant–pathogen interface underpins pathogen recognition

CCPortal

DOI	10.1073/pnas.2104997118
	Dynamic localization of a helper NLR at the plant–pathogen interface underpins pathogen recognition
	Duggan C.; Moratto E.; Savage Z.; Hamilton E.; Adachi H.; Wu C.-H.; Leary A.Y.; Tumtas Y.; Rothery S.M.; Maqbool A.; Nohut S.; Martin T.R.; Kamoun S.; Bozkurt T.O.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:34
英文摘要	Plants employ sensor–helper pairs of NLR immune receptors to recognize pathogen effectors and activate immune responses. Yet, the subcellular localization of NLRs pre- and postactivation during pathogen infection remains poorly understood. Here, we show that NRC4, from the “NRC” solanaceous helper NLR family, undergoes dynamic changes in subcellular localization by shuttling to and from the plant–pathogen haustorium interface established during infection by the Irish potato famine pathogen Phytophthora infestans. Specifically, prior to activation, NRC4 accumulates at the extrahaustorial membrane (EHM), presumably to mediate response to perihaustorial effectors that are recognized by NRC4-dependent sensor NLRs. However, not all NLRs accumulate at the EHM, as the closely related helper NRC2 and the distantly related ZAR1 did not accumulate at the EHM. NRC4 required an intact N-terminal coiled-coil domain to accumulate at the EHM, whereas the functionally conserved MADA motif implicated in cell death activation and membrane insertion was dispensable for this process. Strikingly, a constitutively autoactive NRC4 mutant did not accumulate at the EHM and showed punctate distribution that mainly associated with the plasma membrane, suggesting that postactivation, NRC4 may undergo a conformation switch to form clusters that do not preferentially associate with the EHM. When NRC4 is activated by a sensor NLR during infection, however, NRC4 forms puncta mainly at the EHM and, to a lesser extent, at the plasma membrane. We conclude that following activation at the EHM, NRC4 may spread to other cellular membranes from its primary site of activation to trigger immune responses. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Cell biology; Helper NLR; Host–microbe interactions; Plant disease resistance
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238819
作者单位	Department of Life Sciences, Imperial College, London, SW7 2AZ, United Kingdom; The Sainsbury Laboratory, University of East Anglia, Norwich, NR4 7UH, United Kingdom; Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, 630-0192, Japan; Institute of Plant and Microbial Biology, Academia Sinica, Taipei, 11529, Taiwan
推荐引用方式 GB/T 7714	Duggan C.,Moratto E.,Savage Z.,等. Dynamic localization of a helper NLR at the plant–pathogen interface underpins pathogen recognition[J],2021,118(34).
APA	Duggan C..,Moratto E..,Savage Z..,Hamilton E..,Adachi H..,...&Bozkurt T.O..(2021).Dynamic localization of a helper NLR at the plant–pathogen interface underpins pathogen recognition.Proceedings of the National Academy of Sciences of the United States of America,118(34).
MLA	Duggan C.,et al."Dynamic localization of a helper NLR at the plant–pathogen interface underpins pathogen recognition".Proceedings of the National Academy of Sciences of the United States of America 118.34(2021).