气候变化领域集成服务门户(CCPortal): Amyloid particles facilitate surface-catalyzed cross-seeding by acting as promiscuous nanoparticles

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DOI	10.1073/pnas.2104148118
	Amyloid particles facilitate surface-catalyzed cross-seeding by acting as promiscuous nanoparticles
	Koloteva-Levine N.; Aubrey L.D.; Marchante R.; Purton T.J.; Hiscock J.R.; Tuite M.F.; Xue W.-F.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:36
英文摘要	Amyloid seeds are nanometer-sized protein particles that accelerate amyloid assembly as well as propagate and transmit the amyloid protein conformation associated with a wide range of protein misfolding diseases. However, seeded amyloid growth through templated elongation at fibril ends cannot explain the full range of molecular behaviors observed during cross-seeded formation of amyloid by heterologous seeds. Here, we demonstrate that amyloid seeds can accelerate amyloid formation via a surface catalysis mechanism without propagating the specific amyloid conformation associated with the seeds. This type of seeding mechanism is demonstrated through quantitative characterization of the crossseeded assembly reactions involving two nonhomologous and unrelated proteins: The human Aβ42 peptide and the yeast prion- forming protein Sup35NM. Our results demonstrate experimental approaches to differentiate seeding by templated elongation from nontemplated amyloid seeding and rationalize the molecular mechanism of the cross-seeding phenomenon as a manifestation of the aberrant surface activities presented by amyloid seeds as nanoparticles. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Amyloid β peptide; Atomic force microscopy; Protein aggregation and assembly; Saccharomyces cerevisiae; Sup35 yeast prion protein
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238809
作者单位	Kent Fungal Group, School of Biosciences, University of Kent, Canterbury, CT2 7NJ, United Kingdom; School of Physical Sciences, University of Kent, Canterbury, CT2 7NJ, United Kingdom
推荐引用方式 GB/T 7714	Koloteva-Levine N.,Aubrey L.D.,Marchante R.,et al. Amyloid particles facilitate surface-catalyzed cross-seeding by acting as promiscuous nanoparticles[J],2021,118(36).
APA	Koloteva-Levine N..,Aubrey L.D..,Marchante R..,Purton T.J..,Hiscock J.R..,...&Xue W.-F..(2021).Amyloid particles facilitate surface-catalyzed cross-seeding by acting as promiscuous nanoparticles.Proceedings of the National Academy of Sciences of the United States of America,118(36).
MLA	Koloteva-Levine N.,et al."Amyloid particles facilitate surface-catalyzed cross-seeding by acting as promiscuous nanoparticles".Proceedings of the National Academy of Sciences of the United States of America 118.36(2021).