气候变化领域集成服务门户(CCPortal): Local thermodynamics govern formation and dissolution of Caenorhabditis elegans P granule condensates

CCPortal

DOI	10.1073/pnas.2102772118
	Local thermodynamics govern formation and dissolution of Caenorhabditis elegans P granule condensates
	Fritsch A.W.; Diaz-Delgadillo A.F.; Adame-Arana O.; Hoege C.; Mittasch M.; Kreysing M.; Leaver M.; Hyman A.A.; Julicher F.; Weber C.A.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:37
英文摘要	Membraneless compartments, also known as condensates, provide chemically distinct environments and thus spatially organize the cell. A well-studied example of condensates is P granules in the roundworm Caenorhabditis elegans that play an important role in the development of the germline. P granules are RNArich protein condensates that share the key properties of liquid droplets such as a spherical shape, the ability to fuse, and fast diffusion of their molecular components. An outstanding question is to what extent phase separation at thermodynamic equilibrium is appropriate to describe the formation of condensates in an active cellular environment. To address this question, we investigate the response of P granule condensates in living cells to temperature changes. We observe that P granules dissolve upon increasing the temperature and recondense upon lowering the temperature in a reversible manner. Strikingly, this temperature response can be captured by in vivo phase diagrams that are well described by a Flory-Huggins model at thermodynamic equilibrium. This finding is surprising due to active processes in a living cell. To address the impact of such active processes on intracellular phase separation, we discuss temperature heterogeneities. We show that, for typical estimates of the density of active processes, temperature represents a well-defined variable and that mesoscopic volume elements are at local thermodynamic equilibrium. Our findings provide strong evidence that P granule assembly and disassembly are governed by phase separation based on local thermal equilibria where the nonequilibrium nature of the cytoplasm is manifested on larger scales. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	In vivo phase diagram; P granules; Phase separation; Temperature
语种	英语
scopus关键词	animal cell; animal experiment; animal model; article; Caenorhabditis elegans; cytoplasm; dissolution; in vivo study; nonhuman; phase separation; temperature sensitivity; thermodynamics
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238802
作者单位	Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, 01307, Germany; Max Planck Institute for Evolutionary Biology, Plon, 24306, Germany; Division Biological Physics, Max Planck Institute for the Physics of Complex Systems, Dresden, 01187, Germany; Department of Chemical and Biological Physics, Weizmann Institute of Science, Rehovot, 76100, Israel; Dewpoint Therapeutics GmbH, Dresden, 01307, Germany; Center for Systems Biology Dresden, Dresden, 01307, Germany; Cluster of Excellence Physics of Life, Technische Universität Dresden, Dresden, 01307, Germany
推荐引用方式 GB/T 7714	Fritsch A.W.,Diaz-Delgadillo A.F.,Adame-Arana O.,et al. Local thermodynamics govern formation and dissolution of Caenorhabditis elegans P granule condensates[J],2021,118(37).
APA	Fritsch A.W..,Diaz-Delgadillo A.F..,Adame-Arana O..,Hoege C..,Mittasch M..,...&Weber C.A..(2021).Local thermodynamics govern formation and dissolution of Caenorhabditis elegans P granule condensates.Proceedings of the National Academy of Sciences of the United States of America,118(37).
MLA	Fritsch A.W.,et al."Local thermodynamics govern formation and dissolution of Caenorhabditis elegans P granule condensates".Proceedings of the National Academy of Sciences of the United States of America 118.37(2021).