气候变化领域集成服务门户(CCPortal): Distinct roles of nuclear basket proteins in directing the passage of mRNA through the nuclear pore

CCPortal

DOI	10.1073/pnas.2015621118
	Distinct roles of nuclear basket proteins in directing the passage of mRNA through the nuclear pore
	Li Y.; Aksenova V.; Tingey M.; Yu J.; Ma P.; Arnaoutov A.; Chen S.; Dasso M.; Yang W.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:37
英文摘要	The in vivo characterization of the exact copy number and the specific function of each composite protein within the nuclear pore complex (NPC) remains both desirable and challenging. Through the implementation of live-cell high-speed super-resolution singlemolecule microscopy, we first quantified the native copies of nuclear basket (BSK) proteins (Nup153, Nup50, and Tpr) prior to knocking them down in a highly specific manner via an auxin-inducible degron strategy. Second, we determined the specific roles that BSK proteins play in the nuclear export kinetics ofmodelmessenger RNA (mRNA) substrates. Finally, the three-dimensional (3D) nuclear export routes of these mRNA substrates through native NPCs in the absence of specific BSK proteins were obtained and further validated via postlocalization computational simulations. We found that these BSK proteins possess the stoichiometric ratio of 1:1:1 and play distinct roles in the nuclear export of mRNAs within live cells. The absence of Tpr from the NPC predominantly reduces the probability of nuclearmRNAs entering the NPC for export. Complete depletion of Nup153 and Nup50 results in an mRNA nuclear export efficiency decrease of approximately four folds. mRNAs can gain their maximum successful export efficiency as the copy number of Nup153 increased from zero to only half the full complement natively within the NPC. Lastly, the absence of Tpr or Nup153 seems to alter the 3D export routes of mRNAs as they pass through the NPC. However, the removal of Nup50 alone has almost no impact upon mRNA export route and kinetics. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	3D super-resolution microscopy; NPC stoichiometry; Nucleocytoplasmic transport
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238801
作者单位	Department of Biology, Temple University, Philadelphia, PA 19122, United States; Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20847, United States
推荐引用方式 GB/T 7714	Li Y.,Aksenova V.,Tingey M.,et al. Distinct roles of nuclear basket proteins in directing the passage of mRNA through the nuclear pore[J],2021,118(37).
APA	Li Y..,Aksenova V..,Tingey M..,Yu J..,Ma P..,...&Yang W..(2021).Distinct roles of nuclear basket proteins in directing the passage of mRNA through the nuclear pore.Proceedings of the National Academy of Sciences of the United States of America,118(37).
MLA	Li Y.,et al."Distinct roles of nuclear basket proteins in directing the passage of mRNA through the nuclear pore".Proceedings of the National Academy of Sciences of the United States of America 118.37(2021).