气候变化领域集成服务门户(CCPortal): SARS-CoV-2 evolution in animals suggests mechanisms for rapid variant selection

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DOI	10.1073/pnas.2105253118
	SARS-CoV-2 evolution in animals suggests mechanisms for rapid variant selection
	Bashor L.; Gagne R.B.; Bosco-Lauth A.M.; Bowen R.A.; Stenglein M.; De Woude S.V.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:44
英文摘要	SARS-CoV-2 spillback from humans into domestic and wild animals has been well documented, and an accumulating number of studies illustrate that human-to-animal transmission is widespread in cats, mink, deer, and other species. Experimental inoculations of cats, mink, and ferrets have perpetuated transmission cycles. We sequenced full genomes of Vero cell-expanded SARS-CoV-2 inoculum and viruses recovered from cats (n = 6), dogs (n = 3), hamsters (n = 3), and a ferret (n = 1) following experimental exposure. Five nonsynonymous changes relative to the USA-WA1/2020 prototype strain were near fixation in the stock used for inoculation but had reverted to wild-type sequences at these sites in dogs, cats, and hamsters within 1- to 3-d postexposure. A total of 14 emergent variants (six in nonstructural genes, six in spike, and one each in orf8 and nucleocapsid) were detected in viruses recovered from animals. This included substitutions in spike residues H69, N501, and D614, which also vary in human lineages of concern. Even though a live virus was not cultured from dogs, substitutions in replicase genes were detected in amplified sequences. The rapid selection of SARS-CoV-2 variants in vitro and in vivo reveals residues with functional significance during host switching. These observations also illustrate the potential for spillback from animal hosts to accelerate the evolution of new viral lineages, findings of particular concern for dogs and cats living in households with COVID-19 patients. More generally, this glimpse into viral host switching reveals the unrealized rapidity and plasticity of viral evolution in experimental animal model systems. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Companion animals; Host adaptation; SARS-CoV-2; Spillover; Viral variants
语种	英语
scopus关键词	adult; amplicon; animal cell; animal experiment; animal model; article; cat; controlled study; dog; female; hamster; host adaptation; household; in vitro study; in vivo study; inoculation; male; Mustela putorius furo; Neovison vison; nonhuman; pet animal; Severe acute respiratory syndrome coronavirus 2; spike; Vero cell line; viral evolution; virus nucleocapsid; virus strain; wild animal; wild type
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238767
作者单位	Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, United States; Department of Pathobiology, Wildlife Futures Program, University of Pennsylvania School of Veterinary Medicine, Kennett Square, PA 19348, United States; Department of Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, United States
推荐引用方式 GB/T 7714	Bashor L.,Gagne R.B.,Bosco-Lauth A.M.,et al. SARS-CoV-2 evolution in animals suggests mechanisms for rapid variant selection[J],2021,118(44).
APA	Bashor L.,Gagne R.B.,Bosco-Lauth A.M.,Bowen R.A.,Stenglein M.,&De Woude S.V..(2021).SARS-CoV-2 evolution in animals suggests mechanisms for rapid variant selection.Proceedings of the National Academy of Sciences of the United States of America,118(44).
MLA	Bashor L.,et al."SARS-CoV-2 evolution in animals suggests mechanisms for rapid variant selection".Proceedings of the National Academy of Sciences of the United States of America 118.44(2021).