气候变化领域集成服务门户(CCPortal): Preventing translational inhibition from ribosomal protein insufficiency by a herpes simplex virus-encoded ribosome-associated protein

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DOI	10.1073/pnas.2025546118
	Preventing translational inhibition from ribosomal protein insufficiency by a herpes simplex virus-encoded ribosome-associated protein
	Vink E.I.; Andrews J.; Duffy C.; Mohr I.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:45
英文摘要	In addition to being required for protein synthesis, ribosomes and ribosomal proteins (RPs) also regulate messenger RNA translation in uninfected and virus-infected cells. By individually depleting 85 RPs using RNA interference, we found that overall protein synthesis in uninfected primary fibroblasts was more sensitive to RP depletion than those infected with herpes simplex virus-1 (HSV-1). Although representative RP depletion (uL3, uS4, uL5) inhibited protein synthesis in cells infected with two different DNA viruses (human cytomegalovirus, vaccinia virus), HSV-1-infected cell protein synthesis unexpectedly endured and required a single virusencoded gene product, VP22. During individual RP insufficiency, VP22-expressing HSV-1 replicated better than a VP22-deficient variant. Furthermore, VP22 promotes polysome accumulation in virusinfected cells when uL3 or ribosome availability is limiting and cosediments with initiating and elongating ribosomes in infected and uninfected cells. This identifies VP22 as a virus-encoded, ribosome-associated protein that compensates for RP insufficiency to support viral protein synthesis and replication. Moreover, it reveals an unanticipated class of virus-encoded, ribosome-associated effectors that reduce the dependence of protein synthesis upon host RPs and broadly support translation during physiological stress such as infection. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	HSV-1 infection; Physiological stress; Ribosomal protein insufficiency; Translational control
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238764
作者单位	Department of Microbiology, New York University School of Medicine, New York, NY 10016, United States; Department of Biological Sciences, University of Alabama, Tuscaloosa, AL 35401, United States; Laura and Isaac Perlmutter Cancer Institute, New York University School of Medicine, New York, NY 10016, United States
推荐引用方式 GB/T 7714	Vink E.I.,Andrews J.,Duffy C.,et al. Preventing translational inhibition from ribosomal protein insufficiency by a herpes simplex virus-encoded ribosome-associated protein[J],2021,118(45).
APA	Vink E.I.,Andrews J.,Duffy C.,&Mohr I..(2021).Preventing translational inhibition from ribosomal protein insufficiency by a herpes simplex virus-encoded ribosome-associated protein.Proceedings of the National Academy of Sciences of the United States of America,118(45).
MLA	Vink E.I.,et al."Preventing translational inhibition from ribosomal protein insufficiency by a herpes simplex virus-encoded ribosome-associated protein".Proceedings of the National Academy of Sciences of the United States of America 118.45(2021).