气候变化领域集成服务门户(CCPortal): The bacterial tyrosine kinase system CpsBCD governs the length of capsule polymers

CCPortal

DOI	10.1073/pnas.2103377118
	The bacterial tyrosine kinase system CpsBCD governs the length of capsule polymers
	Nakamoto R.; Kwan J.M.C.; Chin J.F.L.; Ong H.T.; Flores-Kim J.; Midonet C.; VanNieuwenhze M.S.; Guan X.L.; Sham L.-T.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:45
英文摘要	Many pathogenic bacteria are encased in a layer of capsular polysaccharide (CPS). This layer is important for virulence by masking surface antigens, preventing opsonophagocytosis, and avoiding mucus entrapment. The bacterial tyrosine kinase (BY-kinase) regulates capsule synthesis and helps bacterial pathogens to survive different host niches. BY-kinases autophosphorylate at the C-terminal tyrosine residues upon external stimuli, but the role of phosphorylation is still unclear. Here, we report that the BY-kinase CpsCD is required for growth in Streptococcus pneumoniae. Cells lacking a functional cpsC or cpsD accumulated low molecular weight CPS and lysed because of the lethal sequestration of the lipid carrier undecaprenyl phosphate, resulting in inhibition of peptidoglycan (PG) synthesis. CpsC interacts with CpsD and the polymerase CpsH. CpsD phosphorylation reduces the length of CPS polymers presumably by controlling the activity of CpsC. Finally, pulse-chase experiments reveal the spatiotemporal coordination between CPS and PG synthesis. This coordination is dependent on CpsC and CpsD. Together, our study provides evidence that BY-kinases regulate capsule polymer length by fine-tuning CpsC activity through autophosphorylation. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Bacterial tyrosine kinase; Capsular polysaccharide; Streptococcus pneumoniae
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238759
作者单位	Infectious Diseases Translational Research Programme, Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117545, Singapore; Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore, 308232, Singapore; Mechanobiology Institute, National University of Singapore, Singapore, 117411, Singapore; Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA 02115, United States; Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN 47405, United States
推荐引用方式 GB/T 7714	Nakamoto R.,Kwan J.M.C.,Chin J.F.L.,et al. The bacterial tyrosine kinase system CpsBCD governs the length of capsule polymers[J],2021,118(45).
APA	Nakamoto R..,Kwan J.M.C..,Chin J.F.L..,Ong H.T..,Flores-Kim J..,...&Sham L.-T..(2021).The bacterial tyrosine kinase system CpsBCD governs the length of capsule polymers.Proceedings of the National Academy of Sciences of the United States of America,118(45).
MLA	Nakamoto R.,et al."The bacterial tyrosine kinase system CpsBCD governs the length of capsule polymers".Proceedings of the National Academy of Sciences of the United States of America 118.45(2021).