气候变化领域集成服务门户(CCPortal): Functional genomics and metabolomics advance the ethnobotany of the Samoan traditional medicine "matalafi"

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DOI	10.1073/pnas.2100880118
	Functional genomics and metabolomics advance the ethnobotany of the Samoan traditional medicine "matalafi"
	Molimau-Samasoni S.; Woolner V.H.; Foliga S.T.; Robichon K.; Patel V.; Andreassend S.K.; Sheridan J.P.; Kawa T.T.; Gresham D.; Miller D.; Sinclair D.J.; La Flamme A.C.; Melnik A.V.; Aron A.; Dorrestein P.C.; Atkinson P.H.; Keyzers R.A.; Munkacsi A.B.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:45
英文摘要	The leaf homogenate of Psychotria insularum is widely used in Samoan traditional medicine to treat inflammation associated with fever, body aches, swellings, wounds, elephantiasis, incontinence, skin infections, vomiting, respiratory infections, and abdominal distress. However, the bioactive components and underlying mechanisms of action are unknown. We used chemical genomic analyses in the model organism Saccharomyces cerevisiae (baker's yeast) to identify and characterize an iron homeostasis mechanism of action in the traditional medicine as an unfractionated entity to emulate its traditional use. Bioactivity-guided fractionation of the homogenate identified two flavonol glycosides, rutin and nicotiflorin, each binding iron in an ion-dependent molecular networking metabolomics analysis. Translating results to mammalian immune cells and traditional application, the iron chelator activity of the P. insularum homogenate or rutin decreased proinflammatory and enhanced anti-inflammatory cytokine responses in immune cells. Together, the synergistic power of combining traditional knowledge with chemical genomics, metabolomics, and bioassay-guided fractionation provided molecular insight into a relatively understudied Samoan traditional medicine and developed methodology to advance ethnobotany. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Chemical biology; Genomics; Iron homeostasis; Metabolomics; Traditional medicine
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238758
作者单位	Plant and Postharvest Technologies, Scientific Research Organization of Samoa, Apia, Samoa; School of Biological Sciences, Victoria University of Wellington, Wellington, 6012, New Zealand; Centre for Biodiscovery, Victoria University of Wellington, Wellington, 6012, New Zealand; School of Chemical and Physical Sciences, Victoria University of Wellington, Wellington, 6012, New Zealand; Division of Environment and Conservation, Ministry of Natural Resources and Environment, Apia, Samoa; Centre of Genomic and Systems Biology, New York University, New York, NY 10003, United States; Department of Genetics, Stanford University, Palo Alto, CA 94305, United States; School of Geography, Environmental and Earth Sciences, Victoria University of Wellington, Wellington, 6012, New Zealand; Maurice Wilkins Centre for Molecular Biodiscovery, Victoria University of Wellington, Wellington, 6012, New Zealand; Collaborative Mass Spectrometry Innovation Center, Skaggs School of Pharmacy and Pharmaceutical Sciences, Universi...
推荐引用方式 GB/T 7714	Molimau-Samasoni S.,Woolner V.H.,Foliga S.T.,et al. Functional genomics and metabolomics advance the ethnobotany of the Samoan traditional medicine "matalafi"[J],2021,118(45).
APA	Molimau-Samasoni S..,Woolner V.H..,Foliga S.T..,Robichon K..,Patel V..,...&Munkacsi A.B..(2021).Functional genomics and metabolomics advance the ethnobotany of the Samoan traditional medicine "matalafi".Proceedings of the National Academy of Sciences of the United States of America,118(45).
MLA	Molimau-Samasoni S.,et al."Functional genomics and metabolomics advance the ethnobotany of the Samoan traditional medicine "matalafi"".Proceedings of the National Academy of Sciences of the United States of America 118.45(2021).