气候变化领域集成服务门户(CCPortal): Microevolution in the pansecondary metabolome of Aspergillus flavus and its potential macroevolutionary implications for filamentous fungi

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DOI	10.1073/pnas.2021683118
	Microevolution in the pansecondary metabolome of Aspergillus flavus and its potential macroevolutionary implications for filamentous fungi
	Drott M.T.; Rush T.A.; Satterlee T.R.; Giannone R.J.; Abraham P.E.; Greco C.; Venkatesh N.; Skerker J.M.; Louise Glass N.; Labbé J.L.; Milgroom M.G.; Keller N.P.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:21
英文摘要	Fungi produce a wealth of pharmacologically bioactive secondary metabolites (SMs) from biosynthetic gene clusters (BGCs). It is common practice for drug discovery efforts to treat species’ secondary metabolomes as being well represented by a single or a small number of representative genomes. However, this approach misses the possibility that intraspecific population dynamics, such as adaptation to environmental conditions or local microbiomes, may harbor novel BGCs that contribute to the overall niche breadth of species. Using 94 isolates of Aspergillus flavus, a cosmopolitan model fungus, sampled from seven states in the United States, we dereplicate 7,821 BGCs into 92 unique BGCs. We find that more than 25% of pangenomic BGCs show population-specific patterns of presence/absence or protein divergence. Population-specific BGCs make up most of the accessory-genome BGCs, suggesting that different ecological forces that maintain accessory genomes may be partially mediated by population-specific differences in secondary metabolism. We use ultra-high-performance high-resolution mass spectrometry to confirm that these genetic differences in BGCs also result in chemotypic differences in SM production in different populations, which could mediate ecological interactions and be acted on by selection. Thus, our results suggest a paradigm shift that previously unrealized population-level reservoirs of SM diversity may be of significant evolutionary, ecological, and pharmacological importance. Last, we find that several population-specific BGCs from A. flavus are present in Aspergillus parasiticus and Aspergillus minisclerotigenes and discuss how the microevolutionary patterns we uncover inform macroevolutionary inferences and help to align fungal secondary metabolism with existing evolutionary theory. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	allopatric speciation; comparative genomics; eukaryotic pangenome; population genomics; Secondary metabolism
语种	英语
scopus关键词	Article; controlled study; metabolome; nonhuman
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238730
作者单位	Department of Bacteriology, University of Wisconsin–Madison, Madison, WI 53703, United States; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37830, United States; Department of Genetics, University of Wisconsin–Madison, Madison, WI 53703, United States; Department of Molecular Microbiology, John Innes Centre, Norwich, NR4 7UH, United Kingdom; Department of Medical Microbiology and Immunology, University of Wisconsin–Madison, Madison, WI 53703, United States; Department of Plant Pathology, University of Wisconsin–Madison, Madison, WI 53706, United States; Innovative Genomics Institute, University of California, Berkeley, CA 94720, United States; Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, United States; Environmental Genomics and Systems Biology, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, United States; School of Integrative Plant Science, Cornell University, Ithaca, NY 14853, United States
推荐引用方式 GB/T 7714	Drott M.T.,Rush T.A.,Satterlee T.R.,et al. Microevolution in the pansecondary metabolome of Aspergillus flavus and its potential macroevolutionary implications for filamentous fungi[J],2021,118(21).
APA	Drott M.T..,Rush T.A..,Satterlee T.R..,Giannone R.J..,Abraham P.E..,...&Keller N.P..(2021).Microevolution in the pansecondary metabolome of Aspergillus flavus and its potential macroevolutionary implications for filamentous fungi.Proceedings of the National Academy of Sciences of the United States of America,118(21).
MLA	Drott M.T.,et al."Microevolution in the pansecondary metabolome of Aspergillus flavus and its potential macroevolutionary implications for filamentous fungi".Proceedings of the National Academy of Sciences of the United States of America 118.21(2021).