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DOI	10.1073/pnas.2103378118
	A poplar short-chain dehydrogenase reductase plays a potential key role in biphenyl detoxification
	Contreras Á.; Merino I.; Álvarez E.; Bolonio D.; Ortiz J.-E.; Oñate-Sánchez L.; Gómez L.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:35
英文摘要	Polychlorinated biphenyls (PCBs) are persistent organic pollutants with severe effects on human health and the biosphere. Plant-based remediation offersmany benefits over conventional PCB remediation, but its development has been hampered by our poor understanding of biphenyl metabolism in eukaryotes, among other factors. We report here a major PCB-responsive protein in poplar, a plant model system capable of PCB uptake and translocation. We provide structural and functional evidence that this uncharacterized protein, termed SDR57C, belongs to the heterogeneous short-chain dehydrogenase reductase (SDR) superfamily. Despite sequence divergence, structural modeling hinted at structural and functional similarities between SDR57C and BphB, a central component of the Bph pathway for biphenyl/PCB degradation in aerobic bacteria. By combining gas chromatography/mass spectrometry (GC/MS) profiling with a functional complementation scheme, we found that poplar SDR57C can replace BphB activity in the upper Bph pathway of Pseudomonas furukawaii KF707 and therefore catalyze the oxidation of 2,3-dihydro-2,3-dihydroxybiphenyl (2,3-DHDB) to 2,3-dihydroxybiphenyl (2,3-DHB). Consistent with this biochemical activity, we propose a mechanism of action based on prior quantum studies, general properties of SDR enzymes, and the modeled docking of 2,3-DHDB to the SDR57C-NAD+ complex. The putative detoxifying capacity of SDR57C was substantiated through reverse genetics in Arabidopsis thaliana. Phenotypic characterization of the SDR lines underscored an inducible plant pathway with the potential to catabolize toxic biphenyl derivatives. Partial similarities with aerobic bacterial degradation notwithstanding, real-time messenger RNA quantification indicates the occurrence of plant-specific enzymes and features. Our results may help explain differences in degradative abilities among plant genotypes and also provide elements to improve them. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	PCB; Persistent organic pollutants; Phytoremediation; Populus
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238656
作者单位	Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid, Pozuelo de Alarcón, 28223, Spain; Environmental Studies Group, Escuela Técnica Superior de Ingenieros de Minas y Energía, Universidad Politécnica de Madrid, Madrid, 28003, Spain; Departamento de Sistemas y Recursos Naturales, Escuela Técnica Superior de Ingeniería de Montes, Forestal y del Medio Natural, Universidad Politécnica de Madrid, Madrid, 28040, Spain
推荐引用方式 GB/T 7714	Contreras Á.,Merino I.,Álvarez E.,et al. A poplar short-chain dehydrogenase reductase plays a potential key role in biphenyl detoxification[J],2021,118(35).
APA	Contreras Á..,Merino I..,Álvarez E..,Bolonio D..,Ortiz J.-E..,...&Gómez L..(2021).A poplar short-chain dehydrogenase reductase plays a potential key role in biphenyl detoxification.Proceedings of the National Academy of Sciences of the United States of America,118(35).
MLA	Contreras Á.,et al."A poplar short-chain dehydrogenase reductase plays a potential key role in biphenyl detoxification".Proceedings of the National Academy of Sciences of the United States of America 118.35(2021).