气候变化领域集成服务门户(CCPortal): ENHANCED GRAVITROPISM 2 encodes a STERILE ALPHA MOTIF-containing protein that controls root growth angle in barley and wheat

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DOI	10.1073/pnas.2101526118
	ENHANCED GRAVITROPISM 2 encodes a STERILE ALPHA MOTIF-containing protein that controls root growth angle in barley and wheat
	Kirschner G.K.; Rosignoli S.; Guo L.; Vardanega I.; Imani J.; Altmüller J.; Milner S.G.; Balzano R.; Nagel K.A.; Pflugfelder D.; Forestan C.; Bovina R.; Koller R.; Stöcker T.G.; Mascher M.; Simmonds J.; Uauy C.; Schoof H.; Tuberosa R.; Salvi S.; Hochholdinger F.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:35
英文摘要	The root growth angle defines how roots grow toward the gravity vector and is among the most important determinants of root system architecture. It controls water uptake capacity, nutrient use efficiency, stress resilience, and, as a consequence, yield of crop plants. We demonstrated that the egt2 (enhanced gravitropism 2) mutant of barley exhibits steeper root growth of seminal and lateral roots and an auxin-independent higher responsiveness to gravity compared to wild-type plants. We cloned the EGT2 gene by a combination of bulked-segregant analysis and whole genome sequencing. Subsequent validation experiments by an independent CRISPR/Cas9 mutant allele demonstrated that egt2 encodes a STERILE ALPHA MOTIF domain-containing protein. In situ hybridization experiments illustrated that EGT2 is expressed from the root cap to the elongation zone. We demonstrated the evolutionary conserved role of EGT2 in root growth angle control between barley and wheat by knocking out the EGT2 orthologs in the A and B genomes of tetraploid durum wheat. By combining laser capture microdissection with RNA sequencing, we observed that seven expansin genes were transcriptionally down-regulated in the elongation zone. This is consistent with a role of EGT2 in this region of the root where the effect of gravity sensing is executed by differential cell elongation. Our findings suggest that EGT2 is an evolutionary conserved regulator of root growth angle in barley and wheat that could be a valuable target for root-based crop improvement strategies in cereals. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Barley; CRISPR/Cas9; EGT2; Gravitropism; Root angle
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238654
作者单位	Institute of Crop Sciences and Resource Conservation, Crop Functional Genomics, University of Bonn, Bonn, 53113, Germany; Department of Agricultural and Food Sciences, University of Bologna, Bologna, 40127, Italy; Institute of Phytopathology, Research Centre for BioSystems, Land Use and Nutrition, Justus-Liebig-University Giessen, Giessen, 35392, Germany; Cologne Center for Genomics, University of Cologne, Cologne, 50931, Germany; Leibniz Institute of Plant Genetics and Crop Plant Research Gatersleben, Seeland, 06466, Germany; Institute of Bio- and Geosciences, IBG-2: Plant Sciences, Forschungszentrum Juelich GmbH, Jülich, 52425, Germany; Institute of Crop Sciences and Resource Conservation, Crop Bioinformatics, University of Bonn, Bonn, 53115, Germany; German Centre for Integrative Biodiversity Research Halle-Jena-Leipzig, Leipzig, 04103, Germany; John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, United Kingdom
推荐引用方式 GB/T 7714	Kirschner G.K.,Rosignoli S.,Guo L.,et al. ENHANCED GRAVITROPISM 2 encodes a STERILE ALPHA MOTIF-containing protein that controls root growth angle in barley and wheat[J],2021,118(35).
APA	Kirschner G.K..,Rosignoli S..,Guo L..,Vardanega I..,Imani J..,...&Hochholdinger F..(2021).ENHANCED GRAVITROPISM 2 encodes a STERILE ALPHA MOTIF-containing protein that controls root growth angle in barley and wheat.Proceedings of the National Academy of Sciences of the United States of America,118(35).
MLA	Kirschner G.K.,et al."ENHANCED GRAVITROPISM 2 encodes a STERILE ALPHA MOTIF-containing protein that controls root growth angle in barley and wheat".Proceedings of the National Academy of Sciences of the United States of America 118.35(2021).