气候变化领域集成服务门户(CCPortal): Releasing incompatible males drives strong suppression across populations of wild and Wolbachia-carrying Aedes aegypti in Australia

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DOI	10.1073/pnas.2106828118
	Releasing incompatible males drives strong suppression across populations of wild and Wolbachia-carrying Aedes aegypti in Australia
	Beebe N.W.; Pagendam D.; Trewin B.J.; Boomer A.; Bradford M.; Ford A.; Liddington C.; Bondarenco A.; de Barro P.J.; Gilchrist J.; Paton C.; Staunton K.M.; Johnson B.; Maynard A.J.; Devine G.J.; Hugo L.E.; Rasic G.; Cook H.; Massaro P.; Snoad N.; Crawford J.E.; White B.J.; Xi Z.; Ritchie S.A.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:41
英文摘要	Releasing sterile or incompatible male insects is a proven method of population management in agricultural systems with the potential to revolutionize mosquito control. Through a collaborative venture with the “Debug” Verily Life Sciences team, we assessed the incompatible insect technique (IIT) with the mosquito vector Aedes aegypti in northern Australia in a replicated treatment control field trial. Backcrossing a US strain of Ae. aegypti carrying Wolbachia wAlbB from Aedes albopictus with a local strain, we generated a wAlbB2-F4 strain incompatible with both the wild-type (no Wolbachia) and wMel-Wolbachia Ae. aegypti now extant in North Queensland. The wAlbB2-F4 strain was manually mass reared with males separated from females using Verily sex-sorting technologies to obtain no detectable female contamination in the field. With community consent, we delivered a total of three million IIT males into three isolated landscapes of over 200 houses each, releasing ∼50 males per house three times a week over 20 wk. Detecting initial overflooding ratios of between 5:1 and 10:1, strong population declines well beyond 80% were detected across all treatment landscapes when compared to controls. Monitoring through the following season to observe the ongoing effect saw one treatment landscape devoid of adult Ae. aegypti early in the season. A second landscape showed reduced adults, and the third recovered fully. These encouraging results in suppressing both wild-type and wMel-Ae. aegypti confirms the utility of bidirectional incompatibility in the field setting, show the IIT to be robust, and indicate that the removal of this arbovirus vector from human-occupied landscapes may be achievable. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Aedes aegypti; Arbovirus vector; Biological control; Incompatible insect technology; Vector control
语种	英语
scopus关键词	adult; Aedes aegypti; Aedes albopictus; Arbovirus; article; backcrossing; bacterial strain; biological pest control; biomedicine; contamination; controlled study; female; field study; human; major clinical study; male; mosquito vector; nonhuman; Queensland; season; vector control; wild type; Wolbachia
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238623
作者单位	School of Biological Sciences, University of Queensland, Brisbane, QLD 4072, Australia; Commonwealth Scientific and Industrial Research Organisation, Brisbane, QLD 4102, Australia; College of Public Health, Medical and Veterinary Sciences, James Cook University, Smithfield, QLD 4878, Australia; Australian Institute of Tropical Health and Medicine, James Cook University, Smithfield, QLD 4878, Australia; QIMR Berghofer Medical Research Institute, Brisbane, QLD 4029, Australia; Creative Communicator, Cairns, QLD 4870, Australia; Verily Life Sciences, South San Francisco, CA 94080, United States; Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, United States
推荐引用方式 GB/T 7714	Beebe N.W.,Pagendam D.,Trewin B.J.,et al. Releasing incompatible males drives strong suppression across populations of wild and Wolbachia-carrying Aedes aegypti in Australia[J],2021,118(41).
APA	Beebe N.W..,Pagendam D..,Trewin B.J..,Boomer A..,Bradford M..,...&Ritchie S.A..(2021).Releasing incompatible males drives strong suppression across populations of wild and Wolbachia-carrying Aedes aegypti in Australia.Proceedings of the National Academy of Sciences of the United States of America,118(41).
MLA	Beebe N.W.,et al."Releasing incompatible males drives strong suppression across populations of wild and Wolbachia-carrying Aedes aegypti in Australia".Proceedings of the National Academy of Sciences of the United States of America 118.41(2021).