气候变化领域集成服务门户(CCPortal): A multi-omics approach to lignocellulolytic enzyme discovery reveals a new ligninase activity from Parascedosporium putredinis NO1

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DOI	10.1073/pnas.2008888118
	A multi-omics approach to lignocellulolytic enzyme discovery reveals a new ligninase activity from Parascedosporium putredinis NO1
	Oates N.C.; Abood A.; Schirmacher A.M.; Alessi A.M.; Bird S.M.; Bennett J.P.; Leadbeater D.R.; Li Y.; Dowle A.A.; Liu S.; Tymokhin V.I.; Ralph J.; McQueen-Mason S.J.; Bruce N.C.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:18
英文摘要	Lignocellulose, the structural component of plant cells, is a major agricultural byproduct and the most abundant terrestrial source of biopolymers on Earth. The complex and insoluble nature of lignocellulose limits its conversion into value-added commodities, and currently, efficient transformation requires expensive pretreatments and high loadings of enzymes. Here, we report on a fungus from the Parascedosporium genus, isolated from a wheat-straw composting community, that secretes a large and diverse array of carbohydrate-active enzymes (CAZymes) when grown on lignocellulosic substrates. We describe an oxidase activity that cleaves the major β-ether units in lignin, thereby releasing the flavonoid tricin from monocot lignin and enhancing the digestion of lignocellulose by polysaccharidase mixtures. We show that the enzyme, which holds potential for the biorefining industry, is widely distributed among lignocellulose-degrading fungi from the Sordariomycetes phylum. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Lignin; Lignocellulose; Parascedosporium putredinis; Tricin; β-etherase
语种	英语
scopus关键词	lignin; lignin peroxidase; Article; biomass; composting; controlled study; enzyme activity; fungal community; fungus; multiomics; nonhuman; Parascedosporium putredinis; priority journal; protein cleavage
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238588
作者单位	Centre for Novel Agricultural Products, Department of Biology, University of York, York, YO10 5DD, United Kingdom; Bioscience Technology Facility, Department of Biology, University of York, York, YO10 5DD, United Kingdom; Department of Biochemistry, University of Wisconsin, Madison, WI 53726, United States; Department of Energy's Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin, Madison, WI 53726, United States
推荐引用方式 GB/T 7714	Oates N.C.,Abood A.,Schirmacher A.M.,et al. A multi-omics approach to lignocellulolytic enzyme discovery reveals a new ligninase activity from Parascedosporium putredinis NO1[J],2021,118(18).
APA	Oates N.C..,Abood A..,Schirmacher A.M..,Alessi A.M..,Bird S.M..,...&Bruce N.C..(2021).A multi-omics approach to lignocellulolytic enzyme discovery reveals a new ligninase activity from Parascedosporium putredinis NO1.Proceedings of the National Academy of Sciences of the United States of America,118(18).
MLA	Oates N.C.,et al."A multi-omics approach to lignocellulolytic enzyme discovery reveals a new ligninase activity from Parascedosporium putredinis NO1".Proceedings of the National Academy of Sciences of the United States of America 118.18(2021).