气候变化领域集成服务门户(CCPortal): Intranasal vaccination with influenza HA/GO-PEI nanoparticles provides immune protection against homo-and heterologous strains

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DOI	10.1073/pnas.2024998118
	Intranasal vaccination with influenza HA/GO-PEI nanoparticles provides immune protection against homo-and heterologous strains
	Dong C.; Wang Y.; Gonzalez G.X.; Ma Y.; Song Y.; Wang S.; Kang S.-M.; Compans R.W.; Wang B.-Z.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:19
英文摘要	Intranasal (i.n.) immunization is a promising vaccination route for infectious respiratory diseases such as influenza. Recombinant protein vaccines can overcome the safety concerns and long production phase of virus-based influenza vaccines. However, soluble protein vaccines are poorly immunogenic if administered by an i.n. route. Here, we report that polyethyleneimine-functionalized graphene oxide nanoparticles (GP nanoparticles) showed high antigen-loading capacities and superior immunoenhancing properties. Via a facile electrostatic adsorption approach, influenza hemagglutinin (HA) was incorporated into GP nanoparticles and maintained structural integrity and antigenicity. The resulting GP nanoparticles enhanced antigen internalization and promoted inflammatory cytokine production and JAWS II dendritic cell maturation. Compared with soluble HA, GP nanoparticle formulations induced significantly enhanced and cross-reactive immune responses at both systemic sites and mucosal surfaces in mice after i.n. immunization. In the absence of any additional adjuvant, the GP nanoparticle significantly boosted antigen-specific humoral and cellular immune responses, comparable to the acknowledged potent mucosal immunomodulator CpG. The robust immune responses conferred immune protection against challenges by homologous and heterologous viruses. Additionally, the solid self-adjuvant effect of GP nanoparticles may mask the role of CpG when coincorporated. In the absence of currently approved mucosal adjuvants, GP nanoparticles can be developed into potent i.n. influenza vaccines, providing broad protection. With versatility and flexibility, the GP nanoplatform can be easily adapted for constructing mucosal vaccines for different respiratory pathogens. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Graphene oxide nanoparticles; Homologous and heterologous protection; Immunoenhancing effect; Intranasal vaccination; Recombinant protein vaccines
语种	英语
scopus关键词	graphene oxide; Influenza virus hemagglutinin; interleukin 12; interleukin 6; polyethyleneimine; tumor necrosis factor; adsorption; animal cell; animal experiment; animal model; animal tissue; antigen specificity; antigenicity; Article; cell maturation; cellular immunity; controlled study; cross reaction; cytokine production; dendritic cell; drug efficacy; drug formulation; drug safety; female; humoral immunity; immunomodulation; in vitro study; influenza A (H3N2); Influenza A virus (H3N2); influenza vaccination; mouse; mucosal immunity; nonhuman; virus immunity; virus strain
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238580
作者单位	Center for Inflammation, Immunity and Infection, Institute for Biomedical Sciences, Georgia State University, Atlanta, GA 30302, United States; Department of Microbiology and Immunology, Emory University School of Medicine, Emory University, Atlanta, GA 30322, United States
推荐引用方式 GB/T 7714	Dong C.,Wang Y.,Gonzalez G.X.,et al. Intranasal vaccination with influenza HA/GO-PEI nanoparticles provides immune protection against homo-and heterologous strains[J],2021,118(19).
APA	Dong C..,Wang Y..,Gonzalez G.X..,Ma Y..,Song Y..,...&Wang B.-Z..(2021).Intranasal vaccination with influenza HA/GO-PEI nanoparticles provides immune protection against homo-and heterologous strains.Proceedings of the National Academy of Sciences of the United States of America,118(19).
MLA	Dong C.,et al."Intranasal vaccination with influenza HA/GO-PEI nanoparticles provides immune protection against homo-and heterologous strains".Proceedings of the National Academy of Sciences of the United States of America 118.19(2021).