气候变化领域集成服务门户(CCPortal): Fast and pervasive transcriptomic resilience and acclimation of extremely heat-tolerant coral holobionts from the northern Red Sea

CCPortal

DOI	10.1073/pnas.2023298118
	Fast and pervasive transcriptomic resilience and acclimation of extremely heat-tolerant coral holobionts from the northern Red Sea
	Savary R.; Barshis D.J.; Voolstra C.R.; Cárdenas A.; Evensen N.R.; Banc-Prandi G.; Fine M.; Meibom A.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:19
英文摘要	Corals from the northern Red Sea and Gulf of Aqaba exhibit extreme thermal tolerance. To examine the underlying gene expression dynamics, we exposed Stylophora pistillata from the Gulf of Aqaba to short-term (hours) and long-term (weeks) heat stress with peak seawater temperatures ranging from their maximum monthly mean of 27 °C (baseline) to 29.5 °C, 32 °C, and 34.5 °C. Corals were sampled at the end of the heat stress as well as after a recovery period at baseline temperature. Changes in coral host and symbiotic algal gene expression were determined via RNA-sequencing (RNA-Seq). Shifts in coral microbiome composition were detected by complementary DNA (cDNA)-based 16S ribosomal RNA (rRNA) gene sequencing. In all experiments up to 32 °C, RNA-Seq revealed fast and pervasive changes in gene expression, primarily in the coral host, followed by a return to baseline gene expression for the majority of coral (>94%) and algal (>71%) genes during recovery. At 34.5 °C, large differences in gene expression were observed with minimal recovery, high coral mortality, and a microbiome dominated by opportunistic bacteria (including Vibrio species), indicating that a lethal temperature threshold had been crossed. Our results show that the S. pistillata holobiont can mount a rapid and pervasive gene expression response contingent on the amplitude and duration of the thermal stress. We propose that the transcriptomic resilience and transcriptomic acclimation observed are key to the extraordinary thermal tolerance of this holobiont and, by inference, of other northern Red Sea coral holobionts, up to seawater temperatures of at least 32 °C, that is, 5 °C above their current maximum monthly mean. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Coral bleaching; Gene expression profiling; Heat stress; Metaorganism; Microbiome
语种	英语
scopus关键词	complementary DNA; sea water; acclimatization; amplicon; Article; cladistics; climate resilience; controlled study; coral; gene expression; gene sequence; gene structure; heat tolerance; microbial community; mortality rate; nonhuman; Red Sea; RNA sequencing; species composition; Stylophora microadriaticum; Stylophora pistillata; temperature measurement; temperature stress; water temperature
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238577
作者单位	Laboratory for Biological Geochemistry, School of Architecture, Civil and Environmental Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, 1015, Switzerland; Department of Biological Sciences, Old Dominion University, Norfolk, VA 23529, United States; Department of Biology, University of Konstanz, Konstanz, 78457, Germany; The Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 52900, Israel; Laboratory for Coral Reef Ecology, Interuniversity Institute for Marine Sciences, Eilat, 88103, Israel; Center for Advanced Surface Analysis, Institute of Earth Sciences, University of Lausanne, Lausanne, CH-1015, Switzerland
推荐引用方式 GB/T 7714	Savary R.,Barshis D.J.,Voolstra C.R.,et al. Fast and pervasive transcriptomic resilience and acclimation of extremely heat-tolerant coral holobionts from the northern Red Sea[J],2021,118(19).
APA	Savary R..,Barshis D.J..,Voolstra C.R..,Cárdenas A..,Evensen N.R..,...&Meibom A..(2021).Fast and pervasive transcriptomic resilience and acclimation of extremely heat-tolerant coral holobionts from the northern Red Sea.Proceedings of the National Academy of Sciences of the United States of America,118(19).
MLA	Savary R.,et al."Fast and pervasive transcriptomic resilience and acclimation of extremely heat-tolerant coral holobionts from the northern Red Sea".Proceedings of the National Academy of Sciences of the United States of America 118.19(2021).