气候变化领域集成服务门户(CCPortal): Low nitrogen conditions accelerate flowering by modulating the phosphorylation state of FLOWERING BHLH 4 in Arabidopsis

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DOI	10.1073/pnas.2022942118
	Low nitrogen conditions accelerate flowering by modulating the phosphorylation state of FLOWERING BHLH 4 in Arabidopsis
	Sanagi M.; Aoyama S.; Kubo A.; Lu Y.; Sato Y.; Ito S.; Abe M.; Mitsuda N.; Ohme-Takagi M.; Kiba T.; Nakagami H.; Rolland F.; Yamaguchi J.; Imaizumi T.; Sato T.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:19
英文摘要	Nitrogen (N) is an essential nutrient that affects multiple plant developmental processes, including flowering. As flowering requires resources to develop sink tissues for reproduction, nutrient availability is tightly linked to this process. Low N levels accelerate floral transition; however, the molecular mechanisms underlying this response are not well understood. Here, we identify the FLOWERING BHLH 4 (FBH4) transcription factor as a key regulator of N-responsive flowering in Arabidopsis. Low N-induced early flowering is compromised in fbh quadruple mutants. We found that FBH4 is a highly phosphorylated protein and that FBH4 phosphorylation levels decrease under low N conditions. In addition, decreased phosphorylation promotes FBH4 nuclear localization and transcriptional activation of the direct target CONSTANS (CO) and downstream florigen FLOWERING LOCUS T (FT) genes. Moreover, we demonstrate that the evolutionarily conserved cellular fuel sensor SNF1-RELATED KINASE 1 (SnRK1), whose kinase activity is down-regulated under low N conditions, directly phosphorylates FBH4. SnRK1 negatively regulates CO and FT transcript levels under high N conditions. Together, these results reveal a mechanism by which N levels may fine-tune FBH4 nuclear localization by adjusting the phosphorylation state to modulate flowering time. In addition to its role in flowering regulation, we also showed that FBH4 was involved in low N-induced up-regulation of nutrient recycling and remobilization-related gene expression. Thus, our findings provide insight into N-responsive growth phase transitions and optimization of plant fitness under nutrient-limited conditions. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Kinase; Nitrogen availability; Phosphorylation; Photoperiodic flowering; Transcription factor
语种	英语
scopus关键词	basic helix loop helix transcription factor; basic helix loop helix transcription factor 4; nitrogen; unclassified drug; Arabidopsis; Article; controlled study; down regulation; enzyme activity; flowering; gene expression; gene targeting; genetic conservation; nonhuman; phase transition; priority journal; process optimization; protein analysis; protein localization; protein phosphorylation; signal transduction; transcription initiation; upregulation
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238570
作者单位	Faculty of Science, Graduate School of Life Science, Hokkaido University, Sapporo, 060-0810, Japan; Graduate School of Science, Kyoto University, Kyoto, 606-8502, Japan; Graduate School of Arts and Sciences, The University of Tokyo, Tokyo, 153-8902, Japan; Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, 305-8566, Japan; Graduate School of Science and Engineering, Saitama University, Saitama, 338-0825, Japan; Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601, Japan; Protein Mass Spectrometry Group, Max Planck Institute for Plant Breeding Research, Cologne, D-50829, Germany; Biology Department, KU Leuven, Heverlee-Leuven, 3001, Belgium; Department of Biology, University of Washington, Seattle, WA 98195-1800, United States
推荐引用方式 GB/T 7714	Sanagi M.,Aoyama S.,Kubo A.,et al. Low nitrogen conditions accelerate flowering by modulating the phosphorylation state of FLOWERING BHLH 4 in Arabidopsis[J],2021,118(19).
APA	Sanagi M..,Aoyama S..,Kubo A..,Lu Y..,Sato Y..,...&Sato T..(2021).Low nitrogen conditions accelerate flowering by modulating the phosphorylation state of FLOWERING BHLH 4 in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America,118(19).
MLA	Sanagi M.,et al."Low nitrogen conditions accelerate flowering by modulating the phosphorylation state of FLOWERING BHLH 4 in Arabidopsis".Proceedings of the National Academy of Sciences of the United States of America 118.19(2021).