气候变化领域集成服务门户(CCPortal): A synthetic C4 shuttle via the β-hydroxyaspartate cycle in C3 plants

CCPortal

DOI	10.1073/pnas.2022307118
	A synthetic C4 shuttle via the β-hydroxyaspartate cycle in C3 plants
	Roell M.-S.; von Borzykowski L.S.; Westhoff P.; Plett A.; Paczia N.; Claus P.; Urte S.; Erb T.J.; Weber A.P.M.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:21
英文摘要	Plants depend on the enzyme ribulose-1,5-bisphosphate carboxylase/ oxygenase (Rubisco) for CO2 fixation. However, especially in C3 plants, photosynthetic yield is reduced by formation of 2-phosphoglycolate, a toxic oxygenation product of Rubisco, which needs to be recycled in a high-flux–demanding metabolic process called photorespiration. Canonical photorespiration dissipates energy and causes carbon and nitrogen losses. Reducing photorespiration through carbon-concentrating mechanisms, such as C4 photosynthesis, or bypassing photorespiration through metabolic engineering is expected to improve plant growth and yield. The β-hydroxyaspartate cycle (BHAC) is a recently described microbial pathway that converts glyoxylate, a metabolite of plant photorespiration, into oxaloacetate in a highly efficient carbon-, nitrogen-, and energy-conserving manner. Here, we engineered a functional BHAC in plant peroxisomes to create a photorespiratory bypass that is independent of 3-phosphoglycerate regeneration or decarboxylation of photorespiratory precursors. While efficient oxaloacetate conversion in Arabidopsis thaliana still masks the full potential of the BHAC, nitrogen conservation and accumulation of signature C4 metabolites demonstrate the proof of principle, opening the door to engineering a photorespiration-dependent synthetic carbon–concentrating mechanism in C3 plants. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	C4; crop improvement; photorespiration; Photosynthesis; synthetic biology
语种	英语
scopus关键词	3 hydroxyaspartic acid; 3 phosphoglycerate; carbon; glyceric acid; nitrogen; oxaloacetic acid; unclassified drug; Arabidopsis thaliana; Article; beta hydroxyaspartate cycle; bioaccumulation; bioengineering; C3 plant; C4 plant; chemical phenomena; concentration (parameter); controlled study; crop improvement; decarboxylation; metabolite; nonhuman; peroxisome; photorespiration; photosynthesis; synthetic biology
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238554
作者单位	Institute of Plant Biochemistry, Heinrich Heine University, Düsseldorf, 40225, Germany; Department of Biochemistry and Synthetic Metabolism, Max Planck Institute for Terrestrial Microbiology, Marburg, 35043, Germany; Cluster of Excellence on Plant Science, Heinrich Heine University, Düsseldorf, 40225, Germany; LOEWE Center for Synthetic Microbiology, Philipps-University Marburg, Marburg, 35043, Germany
推荐引用方式 GB/T 7714	Roell M.-S.,von Borzykowski L.S.,Westhoff P.,et al. A synthetic C4 shuttle via the β-hydroxyaspartate cycle in C3 plants[J],2021,118(21).
APA	Roell M.-S..,von Borzykowski L.S..,Westhoff P..,Plett A..,Paczia N..,...&Weber A.P.M..(2021).A synthetic C4 shuttle via the β-hydroxyaspartate cycle in C3 plants.Proceedings of the National Academy of Sciences of the United States of America,118(21).
MLA	Roell M.-S.,et al."A synthetic C4 shuttle via the β-hydroxyaspartate cycle in C3 plants".Proceedings of the National Academy of Sciences of the United States of America 118.21(2021).