气候变化领域集成服务门户(CCPortal): A minimally disruptive method for measuring water potential in planta using hydrogel nanoreporters

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DOI	10.1073/pnas.2008276118
	A minimally disruptive method for measuring water potential in planta using hydrogel nanoreporters
	Jain P.; Liu W.; Zhu S.; Chang C.Y.-Y.; Melkonian J.; Rockwell F.E.; Pauli D.; Sun Y.; Zipfel W.R.; Michele Holbrook N.; Riha S.J.; Gore M.A.; Stroock A.D.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:23
英文摘要	Leaf water potential is a critical indicator of plant water status, integrating soil moisture status, plant physiology, and environmental conditions. There are few tools for measuring plant water status (water potential) in situ, presenting a critical barrier for developing appropriate phenotyping (measurement) methods for crop development and modeling efforts aimed at understanding water transport in plants. Here, we present the development of an in situ, minimally disruptive hydrogel nanoreporter (AquaDust) for measuring leaf water potential. The gel matrix responds to changes in water potential in its local environment by swelling; the distance between covalently linked dyes changes with the reconfiguration of the polymer, leading to changes in the emission spectrum via Förster Resonance Energy Transfer (FRET). Upon infiltration into leaves, the nanoparticles localize within the apoplastic space in the mesophyll; they do not enter the cytoplasm or the xylem. We characterize the physical basis for AquaDust's response and demonstrate its function in intact maize (Zea mays L.) leaves as a reporter of leaf water potential. We use AquaDust to measure gradients of water potential along intact, actively transpiring leaves as a function of water status; the localized nature of the reporters allows us to define a hydraulic model that distinguishes resistances inside and outside the xylem. We also present field measurements with AquaDust through a full diurnal cycle to confirm the robustness of the technique and of our model. We conclude that AquaDust offers potential opportunities for high-throughput field measurements and spatially resolved studies of water relations within plant tissues. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Nanobiosensors; Plant-water relations; Responsive hydrogel; Water potential
语种	英语
scopus关键词	hydrogel; nanoparticle; Article; drought; energy transfer; maize; mesophyll; nonhuman; plant tissue; plant water potential; plant water relation; swelling; vegetative growth; water availability; xylem
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238527
作者单位	Sibley School of Mechanical and Aerospace Engineering, Cornell University, Ithaca, NY 14853, United States; Plant Breeding and Genetics Section, School of Integrative Plant Science, Cornell University, Ithaca, NY 14853, United States; Smith School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853, United States; Soil and Crop Sciences Section, School of Integrative Plant Science, Cornell University, Ithaca, NY 14853, United States; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, United States; Department of Biomedical Engineering, Cornell University, Ithaca, NY 14853, United States; Department of Earth and Atmospheric Sciences, Cornell University, Ithaca, NY 14853, United States; Kavli Institute at Cornell for Nanoscale Science, Cornell University, Ithaca, NY 14853, United States; School of Computer Science and Technology, Wuhan University of Technology, Wuhan, 430070, China; School of Plant Sciences, University of Arizona, T...
推荐引用方式 GB/T 7714	Jain P.,Liu W.,Zhu S.,et al. A minimally disruptive method for measuring water potential in planta using hydrogel nanoreporters[J],2021,118(23).
APA	Jain P..,Liu W..,Zhu S..,Chang C.Y.-Y..,Melkonian J..,...&Stroock A.D..(2021).A minimally disruptive method for measuring water potential in planta using hydrogel nanoreporters.Proceedings of the National Academy of Sciences of the United States of America,118(23).
MLA	Jain P.,et al."A minimally disruptive method for measuring water potential in planta using hydrogel nanoreporters".Proceedings of the National Academy of Sciences of the United States of America 118.23(2021).