气候变化领域集成服务门户(CCPortal): Functional compartmentalization and metabolic separation in a prokaryotic cell

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DOI	10.1073/pnas.2022114118
	Functional compartmentalization and metabolic separation in a prokaryotic cell
	Flechsler J.; Heimerl T.; Huber H.; Rachel R.; Berg I.A.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:25
英文摘要	The prokaryotic cell is traditionally seen as a “bag of enzymes,” yet its organization is much more complex than in this simplified view. By now, various microcompartments encapsulating metabolic enzymes or pathways are known for Bacteria. These microcompartments are usually small, encapsulating and concentrating only a few enzymes, thus protecting the cell from toxic intermediates or preventing unwanted side reactions. The hyperthermophilic, strictly anaerobic Crenarchaeon Ignicoccus hospitalis is an extraordinary organism possessing two membranes, an inner and an energized outer membrane. The outer membrane (termed here outer cytoplasmic membrane) harbors enzymes involved in proton gradient generation and ATP synthesis. These two membranes are separated by an intermembrane compartment, whose function is unknown. Major information processes like DNA replication, RNA synthesis, and protein biosynthesis are located inside the “cytoplasm” or central cytoplasmic compartment. Here, we show by immunogold labeling of ultrathin sections that enzymes involved in autotrophic CO2 assimilation are located in the intermembrane compartment that we name (now) a peripheric cytoplasmic compartment. This separation may protect DNA and RNA from reactive aldehydes arising in the I. hospitalis carbon metabolism. This compartmentalization of metabolic pathways and information processes is unprecedented in the prokaryotic world, representing a unique example of spatiofunctional compartmentalization in the second domain of life. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Archaea \| compartmentalization \| CO2 fixation \| immunogold labeling
语种	英语
scopus关键词	aldehyde; DNA; oxaloacetic acid; reduced nicotinamide adenine dinucleotide; reduced nicotinamide adenine dinucleotide phosphate; RNA; succinic semialdehyde; Article; autotrophy; biochemical analysis; carbon fixation; cell compartmentalization; cellular distribution; controlled study; cytoplasm; DNA replication; Ignicoccus hospitalis; immunogold labeling; nonhuman; prokaryotic cell; protein synthesis; RNA synthesis
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238517
作者单位	Center for Electron Microscopy, Faculty of Natural Sciences III, University of Regensburg, Regensburg, 93053, Germany; Plant Development and ElectronMicroscopy, Department of Biology I, Ludwig Maximilian University of Munich, Planegg-Martinsried, 82152, Germany; LOEWE Research Center for SyntheticMicrobiology (SYNMIKRO), Philipps University of Marburg, Marburg, 35043, Germany; Institute for Microbiology and Archaeal Centre, University ofRegensburg, Regensburg, 93053, Germany; Institute for Molecular Microbiology and Biotechnology, University of Münster, Münster, 48149, Germany
推荐引用方式 GB/T 7714	Flechsler J.,Heimerl T.,Huber H.,et al. Functional compartmentalization and metabolic separation in a prokaryotic cell[J],2021,118(25).
APA	Flechsler J.,Heimerl T.,Huber H.,Rachel R.,&Berg I.A..(2021).Functional compartmentalization and metabolic separation in a prokaryotic cell.Proceedings of the National Academy of Sciences of the United States of America,118(25).
MLA	Flechsler J.,et al."Functional compartmentalization and metabolic separation in a prokaryotic cell".Proceedings of the National Academy of Sciences of the United States of America 118.25(2021).