气候变化领域集成服务门户(CCPortal): Aerobic bacterial methane synthesis

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DOI	10.1073/pnas.2019229118
	Aerobic bacterial methane synthesis
	Wang Q.; Alowaifeer A.; Kerner P.; Balasubramanian N.; Patterson A.; Christian W.; Tarver A.; Dore J.E.; Hatzenpichler R.; Bothner B.; McDermott T.R.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:27
英文摘要	Reports of biogenic methane (CH4) synthesis associated with a range of organisms have steadily accumulated in the literature. This has not happened without controversy and in most cases the process is poorly understood at the gene and enzyme levels. In marine and freshwater environments, CH4 supersaturation of oxic surface waters has been termed the “methane paradox” because biological CH4 synthesis is viewed to be a strictly anaerobic process carried out by O2-sensitive methanogens. Interest in this phenomenon has surged within the past decade because of the importance of understanding sources and sinks of this potent greenhouse gas. In our work on Yellowstone Lake in Yellowstone National Park, we demonstrate microbiological conversion of methylamine to CH4 and isolate and characterize an Acidovorax sp. capable of this activity. Furthermore, we identify and clone a gene critical to this process (encodes pyridoxylamine phosphate-dependent aspartate aminotransferase) and demonstrate that this property can be transferred to Escherichia coli with this gene and will occur as a purified enzyme. This previously unrecognized process sheds light on environmental cycling of CH4, suggesting that O2-insensitive, ecologically relevant aerobic CH4 synthesis is likely of widespread distribution in the environment and should be considered in CH4 modeling efforts. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Aerobic; Bacteria; Glycine betaine; Methane; Methylamine
语种	英语
scopus关键词	aspartate aminotransferase; methane; methylamine; oxygen; pyridoxal 5 phosphate; aerobic bacterium; Article; carbon cycling; catalyst; enzyme purification; Escherichia coli; gene identification; isolation procedure; metabolism; microbial community; molecular cloning; mutational analysis; nitrogen cycling; nonhuman; synthesis; United States
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238490
作者单位	Department of Land Resources and Environmental Sciences, Montana State University, Bozeman, MT 59717, United States; Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59717, United States; US Department of Energy, Joint Genome Institute, Walnut Creek, CA 94720, United States; Institute on Ecosystems, Montana State University, Bozeman, MT 59717, United States
推荐引用方式 GB/T 7714	Wang Q.,Alowaifeer A.,Kerner P.,et al. Aerobic bacterial methane synthesis[J],2021,118(27).
APA	Wang Q..,Alowaifeer A..,Kerner P..,Balasubramanian N..,Patterson A..,...&McDermott T.R..(2021).Aerobic bacterial methane synthesis.Proceedings of the National Academy of Sciences of the United States of America,118(27).
MLA	Wang Q.,et al."Aerobic bacterial methane synthesis".Proceedings of the National Academy of Sciences of the United States of America 118.27(2021).