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DOI10.1073/pnas.2106724118
Minute-scale detection of SARS-CoV-2 using a low-cost biosensor composed of pencil graphite electrodes
de Lima L.F.; Ferreira A.L.; Torres M.D.T.; de Araujo W.R.; de la Fuente-Nunez C.
发表日期2021
ISSN0027-8424
卷号118期号:30
英文摘要COVID-19 has led to over 3.47 million deaths worldwide and continues to devastate primarily middle- and low-income countries. High-frequency testing has been proposed as a potential solution to prevent outbreaks. However, current tests are not sufficiently low-cost, rapid, or scalable to enable broad COVID-19 testing. Here, we describe LEAD (Low-cost Electrochemical Advanced Diagnostic), a diagnostic test that detects severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) within 6.5 min and costs $1.50 per unit to produce using easily accessible and commercially available materials. LEAD is highly sensitive toward SARS-CoV-2 spike protein (limit of detection = 229 fg·mL−1) and displays an excellent performance profile using clinical saliva (100.0% sensitivity, 100.0% specificity, and 100.0% accuracy) and nasopharyngeal/oropharyngeal (88.7% sensitivity, 86.0% specificity, and 87.4% accuracy) samples. No cross-reactivity was detected with other coronavirus or influenza strains. Importantly, LEAD also successfully diagnosed the highly contagious SARS-CoV-2 B.1.1.7 UK variant. The device presents high reproducibility under all conditions tested and preserves its original sensitivity for 5 d when stored at 4 °C in phosphate-buffered saline. Our low-cost and do-it-yourself technology opens new avenues to facilitate high-frequency testing and access to much-needed diagnostic tests in resource-limited settings and low-income communities. © 2021 National Academy of Sciences. All rights reserved.
英文关键词COVID-19 | sensor | point of care | graphite | ACE2
语种英语
scopus关键词coronavirus spike glycoprotein; graphite; spike protein, SARS-CoV-2; chemistry; diagnosis; electrode; genetic procedures; human; metabolism; sensitivity and specificity; Biosensing Techniques; COVID-19; COVID-19 Testing; Electrodes; Graphite; Humans; SARS-CoV-2; Sensitivity and Specificity; Spike Glycoprotein, Coronavirus
来源期刊Proceedings of the National Academy of Sciences of the United States of America (IF:9.58[JCR-2018],10.6[5-Year])
文献类型期刊论文
条目标识符http://gcip.llas.ac.cn/handle/2XKMVOVA/238464
作者单位Machine Biology Group, Departments of Psychiatry and Microbiology, University of Pennsylvania, Philadelphia, PA 19104, United States; Institute for Biomedical Informatics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, United States; Institute for Translational Medicine and Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, United States; Department of Bioengineering, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, PA 19104, United States; Department of Chemical and Biomolecular Engineering, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, PA 19104, United States; Penn Institute for Computational Science, University of Pennsylvania, Philadelphia, PA 19104, United States; Portable Chemical Sensors Lab, Department of Analytical Chemistry, Institute of Chemistry, State University of Campinas, São Paulo, 13083-970, Brazil
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de Lima L.F.,Ferreira A.L.,Torres M.D.T.,et al. Minute-scale detection of SARS-CoV-2 using a low-cost biosensor composed of pencil graphite electrodes[J],2021,118(30).
APA de Lima L.F.,Ferreira A.L.,Torres M.D.T.,de Araujo W.R.,&de la Fuente-Nunez C..(2021).Minute-scale detection of SARS-CoV-2 using a low-cost biosensor composed of pencil graphite electrodes.Proceedings of the National Academy of Sciences of the United States of America,118(30).
MLA de Lima L.F.,et al."Minute-scale detection of SARS-CoV-2 using a low-cost biosensor composed of pencil graphite electrodes".Proceedings of the National Academy of Sciences of the United States of America 118.30(2021).
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