气候变化领域集成服务门户(CCPortal): Outer membrane permeability: Antimicrobials and diverse nutrients bypass porins in Pseudomonas aeruginosa

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DOI	10.1073/pnas.2107644118
	Outer membrane permeability: Antimicrobials and diverse nutrients bypass porins in Pseudomonas aeruginosa
	Ude J.; Tripathi V.; Buyck J.M.; Söderholm S.; Cunrath O.; Fanous J.; Claudi B.; Egli A.; Schleberger C.; Hiller S.; Bumann D.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:31
英文摘要	Gram-negative bacterial pathogens have an outer membrane that restricts entry of molecules into the cell. Water-filled protein channels in the outer membrane, so-called porins, facilitate nutrient uptake and are thought to enable antibiotic entry. Here, we determined the role of porins in a major pathogen, Pseudomonas aeruginosa, by constructing a strain lacking all 40 identifiable porins and 15 strains carrying only a single unique type of porin and characterizing these strains with NMR metabolomics and antimicrobial susceptibility assays. In contrast to common assumptions, all porins were dispensable for Pseudomonas growth in rich medium and consumption of diverse hydrophilic nutrients. However, preferred nutrients with two or more carboxylate groups such as succinate and citrate permeated poorly in the absence of porins. Porins provided efficient translocation pathways for these nutrients with broad and overlapping substrate selectivity while efficiently excluding all tested antibiotics except carbapenems, which partially entered through OprD. Porin-independent permeation of antibiotics through the outer-membrane lipid bilayer was hampered by carboxylate groups, consistent with our nutrient data. Together, these results challenge common assumptions about the role of porins by demonstrating porin-independent permeation of the outer-membrane lipid bilayer as a major pathway for nutrient and drug entry into the bacterial cell. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Antimicrobial resistance; Bacterial outer membrane; Diffusion; Lipid bilayer; Membrane transport
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238451
作者单位	Biozentrum, University of Basel, Basel, CH-4056, Switzerland; University Hospital, University of Basel, Basel, CH-4056, Switzerland; Department of Biomedicine, University of Basel, Basel, CH-4056, Switzerland
推荐引用方式 GB/T 7714	Ude J.,Tripathi V.,Buyck J.M.,et al. Outer membrane permeability: Antimicrobials and diverse nutrients bypass porins in Pseudomonas aeruginosa[J],2021,118(31).
APA	Ude J..,Tripathi V..,Buyck J.M..,Söderholm S..,Cunrath O..,...&Bumann D..(2021).Outer membrane permeability: Antimicrobials and diverse nutrients bypass porins in Pseudomonas aeruginosa.Proceedings of the National Academy of Sciences of the United States of America,118(31).
MLA	Ude J.,et al."Outer membrane permeability: Antimicrobials and diverse nutrients bypass porins in Pseudomonas aeruginosa".Proceedings of the National Academy of Sciences of the United States of America 118.31(2021).