气候变化领域集成服务门户(CCPortal): In vitro turnover numbers do not reflect in vivo activities of yeast enzymes

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DOI	10.1073/pnas.2108391118
	In vitro turnover numbers do not reflect in vivo activities of yeast enzymes
	Chen Y.; Nielsen J.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:32
英文摘要	Turnover numbers (kcat values) quantitatively represent the activity of enzymes, which are mostly measured in vitro. While a few studies have reported in vivo catalytic rates (kapp values) in bacteria, a large-scale estimation of kapp in eukaryotes is lacking. Here, we estimated kapp of the yeast Saccharomyces cerevisiae under diverse conditions. By comparing the maximum kapp across conditions with in vitro kcat we found a weak correlation in log scale of R2 = 0.28, which is lower than for Escherichia coli (R2 = 0.62). The weak correlation is caused by the fact that many in vitro kcat values were measured for enzymes obtained through heterologous expression. Removal of these enzymes improved the correlation to R2 = 0.41 but still not as good as for E. coli, suggesting considerable deviations between in vitro and in vivo enzyme activities in yeast. By parameterizing an enzyme-constrained metabolic model with our kapp dataset we observed better performance than the default model with in vitro kcat in predicting proteomics data, demonstrating the strength of using the dataset generated here. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Kcat; Metabolism; Proteomics; Saccharomyces cerevisiae; Turnover number
语种	英语
scopus关键词	article; enzyme activity; Escherichia coli; heterologous expression; in vitro study; in vivo study; nonhuman; proteomics; Saccharomyces cerevisiae; turnover number
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238447
作者单位	Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, SE412 96, Sweden; Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, DK2800 Kgs. Lyngby, Denmark; BioInnovation Institute, Copenhagen N, DK2200, Denmark
推荐引用方式 GB/T 7714	Chen Y.,Nielsen J.. In vitro turnover numbers do not reflect in vivo activities of yeast enzymes[J],2021,118(32).
APA	Chen Y.,&Nielsen J..(2021).In vitro turnover numbers do not reflect in vivo activities of yeast enzymes.Proceedings of the National Academy of Sciences of the United States of America,118(32).
MLA	Chen Y.,et al."In vitro turnover numbers do not reflect in vivo activities of yeast enzymes".Proceedings of the National Academy of Sciences of the United States of America 118.32(2021).