气候变化领域集成服务门户(CCPortal): Methane-derived carbon flows into host-virus networks at different trophic levels in soil

CCPortal

DOI	10.1073/pnas.2105124118
	Methane-derived carbon flows into host-virus networks at different trophic levels in soil
	Lee S.; Sieradzki E.T.; Nicolas A.M.; Walker R.L.; Firestone M.K.; Hazard C.; Nicol G.W.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:32
英文摘要	The concentration of atmospheric methane (CH4) continues to increase with microbial communities controlling soil-atmosphere fluxes. While there is substantial knowledge of the diversity and function of prokaryotes regulating CH4production and consumption, their active interactions with viruses in soil have not been identified. Metagenomic sequencing of soil microbial communities enables identification of linkages between viruses and hosts. However, this does not determine if these represent current or historical interactions nor whether a virus or host are active. In this study, we identified active interactions between individual host and virus populations in situ by following the transfer of assimilated carbon. Using DNA stable-isotope probing combined with metagenomic analyses, we characterized CH4-fueled microbial networks in acidic and neutral pH soils, specifically primary and secondary utilizers, together with the recent transfer of CH4-derived carbon to viruses. A total of 63% of viral contigs from replicated soil incubations contained homologs of genes present in known methylotrophic bacteria. Genomic sequences of 13C-enriched viruses were represented in over one-third of spacers in CRISPR arrays of multiple closely related Methylocystis populations and revealed differences in their history of viral interaction. Viruses infecting nonmethanotrophic methylotrophs and heterotrophic predatory bacteria were also identified through the analysis of shared homologous genes, demonstrating that carbon is transferred to a diverse range of viruses associated with CH4-fueled microbial food networks. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Methanotrophy; Methylotrophy; Predator; Stable isotope probing; Viruses
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238442
作者单位	Environmental Microbial Genomics Group, Laboratoire Ampère, École Centrale de Lyon, CNRS UMR 5005, Université de Lyon, Ecully, 69134, France; Department of Environmental Science, Policy and Management, University of California, Berkeley, CA 94720, United States; Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, United States; School of Rural Land Use, Scotland's Rural College, Aberdeen, AB21 9YA, United Kingdom; Earth Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, United States
推荐引用方式 GB/T 7714	Lee S.,Sieradzki E.T.,Nicolas A.M.,et al. Methane-derived carbon flows into host-virus networks at different trophic levels in soil[J],2021,118(32).
APA	Lee S..,Sieradzki E.T..,Nicolas A.M..,Walker R.L..,Firestone M.K..,...&Nicol G.W..(2021).Methane-derived carbon flows into host-virus networks at different trophic levels in soil.Proceedings of the National Academy of Sciences of the United States of America,118(32).
MLA	Lee S.,et al."Methane-derived carbon flows into host-virus networks at different trophic levels in soil".Proceedings of the National Academy of Sciences of the United States of America 118.32(2021).