气候变化领域集成服务门户(CCPortal): A polyyne toxin produced by an antagonistic bacterium blinds and lyses a Chlamydomonad alga

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DOI	10.1073/pnas.2107695118
	A polyyne toxin produced by an antagonistic bacterium blinds and lyses a Chlamydomonad alga
	Hotter V.; Zopf D.; Kim H.J.; Silge A.; Schmitt M.; Aiyar P.; Fleck J.; Matthäus C.; Hniopek J.; Yan Q.; Loper J.; Sasso S.; Hertweck C.; Popp J.; Mittag M.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:33
英文摘要	Algae are key contributors to global carbon fixation and form the basis of many food webs. In nature, their growth is often supported or suppressed by microorganisms. The bacterium Pseudomonas protegens Pf-5 arrests the growth of the green unicellular alga Chlamydomonas reinhardtii, deflagellates the alga by the cyclic lipopeptide orfamide A, and alters its morphology [P. Aiyar et al., Nat. Commun. 8, 1756 (2017)]. Using a combination of Raman microspectroscopy, genome mining, and mutational analysis, we discovered a polyyne toxin, protegencin, which is secreted by P. protegens, penetrates the algal cells, and causes destruction of the carotenoids of their primitive visual system, the eyespot. Together with secreted orfamide A, protegencin thus prevents the phototactic behavior of C. reinhardtii. A mutant of P. protegens deficient in protegencin production does not affect growth or eyespot carotenoids of C. reinhardtii. Protegencin acts in a direct and destructive way by lysing and killing the algal cells. The toxic effect of protegencin is also observed in an eyeless mutant and with the colony-forming Chlorophyte alga Gonium pectorale. These data reveal a two-pronged molecular strategy involving a cyclic lipopeptide and a conjugated tetrayne used by bacteria to attack select Chlamydomonad algae. In conjunction with the bloom-forming activity of several chlorophytes and the presence of the protegencin gene cluster in over 50 different Pseudomonas genomes [A. J. Mullins et al., bioRxiv [Preprint] (2021). https://www.biorxiv.org/content/10.1101/2021.03.05.433886v1 (Accessed 17 April 2021)], these data are highly relevant to ecological interactions between Chlorophyte algae and Pseudomonadales bacteria. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Chlamydomonas reinhardtii; Eyespot; Microbial interactions; Pseudomonas genome mining; Raman microspectroscopy
语种	英语
scopus关键词	bacterial toxin; carotenoid; lipopeptide; orfamide A; polyyne toxin; protegencin; unclassified drug; algal cell culture; Article; bacterial genome; bacterial secretion system; cell killing; Chlamydomonadales; Chlamydomonas reinhardtii; colony forming unit; controlled study; gene cluster; genetic analysis; Gonium pectorale; green alga; mutational analysis; nonhuman; phototaxis; Pseudomonas protegens; Raman spectrometry; toxin synthesis; visual system
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238433
作者单位	Matthias Schleiden Institute of Genetics, Bioinformatics and Molecular Botany, Friedrich Schiller University Jena, Jena, 07743, Germany; Institute of Physical Chemistry, Friedrich Schiller University Jena, Jena, 07743, Germany; Abbe Center of Photonics, Friedrich Schiller University Jena, Jena, 07743, Germany; Leibniz Institute of Photonic Technology, Jena, 07745, Germany; Leibniz Research Alliance, Leibniz Health Technologies, Jena, 07745, Germany; Department of Biomolecular Chemistry, Leibniz Institute for Natural Product Research and Infection Biology (HKI), Jena, 07745, Germany; Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman, MT 59717, United States; Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331, United States; Institute of Biology, Plant Physiology, Leipzig University, Leipzig, 04103, Germany; Faculty of Biological Sciences, Friedrich Schiller University Jena, Jena, 07743, Germany
推荐引用方式 GB/T 7714	Hotter V.,Zopf D.,Kim H.J.,et al. A polyyne toxin produced by an antagonistic bacterium blinds and lyses a Chlamydomonad alga[J],2021,118(33).
APA	Hotter V..,Zopf D..,Kim H.J..,Silge A..,Schmitt M..,...&Mittag M..(2021).A polyyne toxin produced by an antagonistic bacterium blinds and lyses a Chlamydomonad alga.Proceedings of the National Academy of Sciences of the United States of America,118(33).
MLA	Hotter V.,et al."A polyyne toxin produced by an antagonistic bacterium blinds and lyses a Chlamydomonad alga".Proceedings of the National Academy of Sciences of the United States of America 118.33(2021).