气候变化领域集成服务门户(CCPortal): Enlisting wild grass genes to combat nitrification in wheat farming: A nature-based solution

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DOI	10.1073/pnas.2106595118
	Enlisting wild grass genes to combat nitrification in wheat farming: A nature-based solution
	Subbarao G.V.; Kishii M.; Bozal-Leorri A.; Ortiz-Monasterio I.; Gao X.; Ibba M.I.; Karwat H.; Gonzalez-Moro M.B.; Gonzalez-Murua C.; Yoshihashi T.; Tobita S.; Kommerell V.; Braun H.-J.; Iwanaga M.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:35
英文摘要	Active nitrifiers and rapid nitrification are major contributing factors to nitrogen losses in global wheat production. Suppressing nitrifier activity is an effective strategy to limit N losses from agriculture. Production and release of nitrification inhibitors from plant roots is termed “biological nitrification inhibition” (BNI). Here, we report the discovery of a chromosome region that controls BNI production in “wheat grass” Leymus racemosus (Lam.) Tzvelev, located on the short arm of the “Lr#3Nsb” (Lr#n), which can be transferred to wheat as T3BL.3NsbS (denoted Lr#n-SA), where 3BS arm of chromosome 3B of wheat was replaced by 3NsbS of L. racemosus. We successfully introduced T3BL.3NsbS into the wheat cultivar “Chinese Spring” (CS-Lr#n-SA, referred to as “BNI-CS”), which resulted in the doubling of its BNI capacity. T3BL.3NsbS from BNI-CS was then transferred to several elite high-yielding hexaploid wheat cultivars, leading to near doubling of BNI production in “BNI-MUNAL” and “BNI-ROELFS.” Laboratory incubation studies with root-zone soil from field-grown BNI-MUNAL confirmed BNI trait expression, evident from suppression of soil nitrifier activity, reduced nitrification potential, and N2O emissions. Changes in N metabolism included reductions in both leaf nitrate, nitrate reductase activity, and enhanced glutamine synthetase activity, indicating a shift toward ammonium nutrition. Nitrogen uptake from soil organic matter mineralization improved under low N conditions. Biomass production, grain yields, and N uptake were significantly higher in BNI-MUNAL across N treatments. Grain protein levels and breadmaking attributes were not negatively impacted. Wide use of BNI functions in wheat breeding may combat nitrification in high N input-intensive farming but also can improve adaptation to low N input marginal areas. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	BNI; Genetic improvement; Nitrification inhibition; Nitrogen pollution; Wheat
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238416
作者单位	Crop, Livestock and Environment Division, Japan International Research Center for Agricultural Sciences, Ibaraki, 305-8686, Japan; Global Wheat Program, International Maize and Wheat Improvement Center, Texcoco, 56237, Mexico; Department of Plant Biology and Ecology, University of the Basque Country, Bilbao, E-48080, Spain; College of Bioresources Sciences, Nihon University, Kanagawa, 252-0880, Japan
推荐引用方式 GB/T 7714	Subbarao G.V.,Kishii M.,Bozal-Leorri A.,et al. Enlisting wild grass genes to combat nitrification in wheat farming: A nature-based solution[J],2021,118(35).
APA	Subbarao G.V..,Kishii M..,Bozal-Leorri A..,Ortiz-Monasterio I..,Gao X..,...&Iwanaga M..(2021).Enlisting wild grass genes to combat nitrification in wheat farming: A nature-based solution.Proceedings of the National Academy of Sciences of the United States of America,118(35).
MLA	Subbarao G.V.,et al."Enlisting wild grass genes to combat nitrification in wheat farming: A nature-based solution".Proceedings of the National Academy of Sciences of the United States of America 118.35(2021).