气候变化领域集成服务门户(CCPortal): Bacterial endosymbionts protect beneficial soil fungus from nematode attack

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DOI	10.1073/pnas.2110669118
	Bacterial endosymbionts protect beneficial soil fungus from nematode attack
	Büttner H.; Niehs S.P.; Vandelannoote K.; Cseresnyés Z.; Dose B.; Richter I.; Gerst R.; Figge M.T.; Stinear T.P.; Pidot S.J.; Hertweck C.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:37
英文摘要	Fungi of the genus Mortierella occur ubiquitously in soils where they play pivotal roles in carbon cycling, xenobiont degradation, and promoting plant growth. These important fungi are, however, threatened by micropredators such as fungivorous nematodes, and yet little is known about their protective tactics. We report that Mortierella verticillata NRRL 6337 harbors a bacterial endosymbiont that efficiently shields its host from nematode attacks with anthelmintic metabolites. Microscopic investigation and 16S ribosomal DNA analysis revealed that a previously overlooked bacterial symbiont belonging to the genus Mycoavidus dwells in M. verticillata hyphae. Metabolic profiling of the wild-type fungus and a symbiont-free strain obtained by antibiotic treatment as well as genome analyses revealed that highly cytotoxic macrolactones (CJ-12,950 and CJ- 13,357, syn. necroxime C and D), initially thought to be metabolites of the soil-inhabiting fungus, are actually biosynthesized by the endosymbiont. According to comparative genomics, the symbiont belongs to a new species (Candidatus Mycoavidus necroximicus)with 12% of its 2.2 Mb genome dedicated to natural product biosynthesis, including the modular polyketide-nonribosomal peptide synthetase for necroxime assembly. Using Caenorhabditis elegans and the fungivorous nematode Aphelenchus avenae as test strains, we show that necroximes exert highly potent anthelmintic activities. Effective host protection was demonstrated in cocultures of nematodes with symbiotic and chemically complemented aposymbiotic fungal strains. Image analysis andmathematical quantification of nematodemovement enabled evaluation of the potency. Our work describes a relevant role for endofungal bacteria in protecting fungi against mycophagous nematodes. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Microbial interactions; Natural products; Symbiosis
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238389
作者单位	Department of Biomolecular Chemistry, Leibniz Institute for Natural Product Research and Infection Biology-Hans Knöll Institute (HKI), Jena, 07745, Germany; Department of Microbiology and Immunology, Doherty Institute, Melbourne, 3000, Australia; Applied Systems Biology Research Group, Leibniz Institute for Natural Product Research and Infection Biology-Hans Knöll Institute (HKI), Jena, 07745, Germany; Institute of Microbiology, Faculty of Biological Sciences, Friedrich Schiller University Jena, Jena, 07743, Germany
推荐引用方式 GB/T 7714	Büttner H.,Niehs S.P.,Vandelannoote K.,et al. Bacterial endosymbionts protect beneficial soil fungus from nematode attack[J],2021,118(37).
APA	Büttner H..,Niehs S.P..,Vandelannoote K..,Cseresnyés Z..,Dose B..,...&Hertweck C..(2021).Bacterial endosymbionts protect beneficial soil fungus from nematode attack.Proceedings of the National Academy of Sciences of the United States of America,118(37).
MLA	Büttner H.,et al."Bacterial endosymbionts protect beneficial soil fungus from nematode attack".Proceedings of the National Academy of Sciences of the United States of America 118.37(2021).