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DOI	10.1073/pnas.2106580118
	FAD-dependent C-glycoside–metabolizing enzymes in microorganisms: Screening, characterization, and crystal structure analysis
	Kumano T.; Hori S.; Watanabe S.; Terashita Y.; Yu H.Y.; Hashimoto Y.; Senda T.; Senda M.; Kobayashi M.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:40
英文摘要	C-glycosides have a unique structure, in which an anomeric carbon of a sugar is directly bonded to the carbon of an aglycone skeleton. One of the natural C-glycosides, carminic acid, is utilized by the food, cosmetic, and pharmaceutical industries, for a total of more than 200 tons/y worldwide. However, a metabolic pathway of carminic acid has never been identified. In this study, we isolated the previously unknown carminic acid-catabolizing microorganism and discovered a flavoenzyme “C-glycoside 3-oxidase” named CarA that catalyzes oxidation of the sugar moiety of carminic acid. A Basic Local Alignment Search Tool (BLAST) search demonstrated that CarA homologs were distributed in soil microorganisms but not intestinal ones. In addition to CarA, two CarA homologs were cloned and heterologously expressed, and their biochemical properties were determined. Furthermore, a crystal structure of one homolog was determined. Together with the biochemical analysis, the crystal structure and a mutagenesis analysis of CarA revealed the mechanisms underlying their substrate specificity and catalytic reaction. Our study suggests that CarA and its homologs play a crucial role in the metabolism of C-glycosides in nature. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	C-glycoside; Microorganism; Oxidase
语种	英语
scopus关键词	C glycoside; C glycoside 3 oxidase; carminic acid; flavine adenine nucleotide; glucose; glycoside; O glycoside; oxidoreductase; unclassified drug; analysis; Article; biochemical analysis; biogeochemistry; catalysis; crystal structure; deglycosylation; enzyme activity; enzyme purification; enzyme specificity; gene cluster; hydrogen bond; metabolism; microorganism; mutagenesis analysis; nonhuman; oxidation; screening; soil microflora; structure activity relation
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238361
作者单位	Graduate School of Life and Environmental Sciences, University of Tsukuba, Ibaraki, Tsukuba, 305-8572, Japan; Microbiology Research Center for Sustainability, University of Tsukuba, Ibaraki, Tsukuba, 305-8572, Japan; Structural Biology Research Center, Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK), Ibaraki, Tsukuba, 305-0801, Japan; School of High Energy Accelerator Science, Graduate University for Advanced Studies (SOKENDAI), Ibaraki, Tsukuba, 305-0801, Japan; Faculty of Pure and Applied Sciences, University of Tsukuba, Ibaraki, Tsukuba, 305-8572, Japan
推荐引用方式 GB/T 7714	Kumano T.,Hori S.,Watanabe S.,等. FAD-dependent C-glycoside–metabolizing enzymes in microorganisms: Screening, characterization, and crystal structure analysis[J],2021,118(40).
APA	Kumano T..,Hori S..,Watanabe S..,Terashita Y..,Yu H.Y..,...&Kobayashi M..(2021).FAD-dependent C-glycoside–metabolizing enzymes in microorganisms: Screening, characterization, and crystal structure analysis.Proceedings of the National Academy of Sciences of the United States of America,118(40).
MLA	Kumano T.,et al."FAD-dependent C-glycoside–metabolizing enzymes in microorganisms: Screening, characterization, and crystal structure analysis".Proceedings of the National Academy of Sciences of the United States of America 118.40(2021).