气候变化领域集成服务门户(CCPortal): The missing enzymatic link in syntrophic methane formation from fatty acids

CCPortal

DOI	10.1073/pnas.2111682118
	The missing enzymatic link in syntrophic methane formation from fatty acids
	Agne M.; Estelmann S.; Seelmann C.S.; Kung J.; Wilkens D.; Koch H.-G.; van der Does C.; Albers S.V.; von Ballmoos C.; Simon J.; Boll M.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:40
英文摘要	The microbial production of methane from organic matter is an essential process in the global carbon cycle and an important source of renewable energy. It involves the syntrophic interaction between methanogenic archaea and bacteria that convert primary fermentation products such as fatty acids to the methanogenic substrates acetate, H2, CO2, or formate. While the concept of syntrophic methane formation was developed half a century ago, the highly endergonic reduction of CO2 to methane by electrons derived from β-oxidation of saturated fatty acids has remained hypothetical. Here, we studied a previously noncharacterized membrane-bound oxidoreductase (EMO) from Syntrophus aciditrophicus containing two heme b cofactors and 8-methylmenaquinone as key redox components of the redox loop–driven reduction of CO2 by acyl–coenzyme A (CoA). Using solubilized EMO and proteoliposomes, we reconstituted the entire electron transfer chain from acyl-CoA to CO2 and identified the transfer from a high- to a low-potential heme b with perfectly adjusted midpoint potentials as key steps in syntrophic fatty acid oxidation. The results close our gap of knowledge in the conversion of biomass into methane and identify EMOs as key players of β-oxidation in (methyl)menaquinone-containing organisms. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Diheme oxidoreductase; Methylmenaquinone; Microbial methane formation; Redox loop; Syntrophy
语种	英语
scopus关键词	8 methylmenaquinone; acyl coenzyme A; carbon dioxide; fatty acid; heme; menaquinone; methane; oxidoreductase; proteoliposome; unclassified drug; Article; bacterial membrane; controlled study; cytoplasm; Deltaproteobacteria; electron transport; fatty acid oxidation; metabolism; methanogenesis; nonhuman; oxidation reduction state; solubilization; Syntrophus aciditrophicus; syntrophy
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238359
作者单位	Faculty of Biology–Microbiology, Albert-Ludwigs-University Freiburg, Freiburg, 79104, Germany; Spemann Graduate School of Biology and Medicine, University of Freiburg, Freiburg, 79104, Germany; Microbial Energy Conversion and Biotechnology, Department of Biology, Technical University of Darmstadt, Darmstadt, 64287, Germany; Institute for Biochemistry and Molecular Biology, ZBMZ, Faculty of Medicine, Albert-Ludwigs-University Freiburg, Freiburg, 79104, Germany; Department of Chemistry and Biochemistry, University of Bern, Bern, 3012, Switzerland
推荐引用方式 GB/T 7714	Agne M.,Estelmann S.,Seelmann C.S.,et al. The missing enzymatic link in syntrophic methane formation from fatty acids[J],2021,118(40).
APA	Agne M..,Estelmann S..,Seelmann C.S..,Kung J..,Wilkens D..,...&Boll M..(2021).The missing enzymatic link in syntrophic methane formation from fatty acids.Proceedings of the National Academy of Sciences of the United States of America,118(40).
MLA	Agne M.,et al."The missing enzymatic link in syntrophic methane formation from fatty acids".Proceedings of the National Academy of Sciences of the United States of America 118.40(2021).